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| Term | chromatography technology | ID (Ontology) | MI:0091 (Molecular Interactions) | |||||
| Definition | Used to separate and/or analyse complex mixtures. The components to be separated are distributed between two phases: a stationary phase (bed) and a mobile phase which percolates through the stationary bed. The nature of the two phases determines the separation criteria exploited by the column such as affinity, ionic charges, size or hydrophobicity of the molecules under analysis. Each type of column can be implemented with the mobile phase under atmospheric or high pressure condition. In this later case columns are designated as High Pressure Liquid Chromatography (HPLC).[ PubMed:14755292 ] | |||||||
| Also Known As | "chromatography" ; "column chromatography" | |||||||
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experimental interaction detection |__biochemical |__chromatography technology 13509 rec. |__affinity chromatography technology 13331 rec. | |__coimmunoprecipitation(+) 11530 rec. | |__luminescence based mammalian interactome mapping 6 rec. | |__pull down(+) 3066 rec. | |__tandem affinity purification 144 rec. |__filter trap assay 1 rec. |__ion exchange chromatography 197 rec. |__molecular sieving 528 rec. |__reverse phase chromatography |
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| Is a | biochemical | ||
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