FB2026_02 , released June 18, 2026

CV Term Report

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General Information

Term

blue native page

ID (Ontology)

MI:0276 (Molecular Interactions)

Definition

Blue native PAGE (BN-PAGE) permits a high-resolution separation of multi-protein complexes under native conditions. Blue native (BN)-PAGE is a charge shift method, in which the electrophoretic mobility of a complex is determined by the negative charge of the bound Coomassie dye and the size and shape of the complex. Coomassie does not act as a detergent and preserves the structure of complexes. Importantly, the resolution of BN-PAGE is much higher than that of other methods such as gel filtration or sucrose-gradient ultracentrifugation. Combined with other pre-purifications or dialysis steps this method permits the analysis of multi-protein complexes of whole cellular lysates by BN-PAGE.[ PubMed:14665681 ]

Also Known As

"bn-page"

Comment

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Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
blue native page (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  comigration in gel electrophoresis
   |__comigration in non denaturing gel electrophoresis
       |__blue native page  197 rec.

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Is a	comigration in non denaturing gel electrophoresis
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Synonyms & Secondary IDs
Synonyms
	"bn-page" EXACT PSI-MI-short
Secondary IDs

External Crossreferences & Linkouts