FB2026_03 , released September 17, 2026
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Term rna three hybrid ID (Ontology) MI:0438 (Molecular Interactions)
Definition In vivo reconstruction of specific RNA-proteins interactions. The DNA binding and transcription activator domains of GAL4 are brought together via the interaction of recombinant RNA. The first hybrid protein contains the DNA binding domain of GAL4 fused to RevM10 (a mutated RNA binding protein of HIV-1 that binds specifically to the Rev responsive element RRE of the env gene). A recombinant RNA contains the RRE sequence and a target RNA sequence X. The second hybrid protein contains the activation domain of GAL4 fused to protein Y tested for its ability to bind the target RNA X. If this interaction occurs the three hybrid reconstructs GAL4 and the transcription of a reporter gene is activated.[ PubMed:12162957 PubMed:8972875 ]
Also Known As"rna 3-hybrid" ; "rna tri hybrid" ; "rna-three hybrid" (for all, see Synonyms field below)
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 rna three hybrid (all annotations which use CV term, excluding "NOT" statements)
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  transcriptional complementation assay
   |__three hybrid
       |__rna three hybrid  21 rec.
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  • "rna 3-hybrid" EXACT PSI-MI-alternate
    "rna tri hybrid" EXACT PSI-MI-alternate
    "rna-three hybrid" EXACT PSI-MI-alternate
    "Three hybrid system" EXACT PSI-MI-alternate
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