FB2026_03 , released September 17, 2026

CV Term Report

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General Information

Term

primer extension assay

ID (Ontology)

MI:2203 (Molecular Interactions)

Definition

Primer extension can be used to determine the 3' end of a given sequence of RNA. This technique requires a radiolabelled primer which is complementary to a region near the 3' end of the RNA. The primer is allowed to anneal to the RNA and reverse transcriptase is used to synthesize a strand of DNA from the RNA until it reaches the 5' end of the RNA. By denaturing the hybrid and using the extended primer DNA strand as a marker on an electrophoretic gel, it is possible to determine the transcriptional start site.[ PubMed:23378648 ]

Also Known As

"primer extension assay"

Comment

Links to External Ontologies

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Interaction Groups
primer extension assay (all annotations which use CV term, excluding "NOT" statements)

Spanning Tree (Parents/Children)

  partial nucleotide sequence identification
   |__partial RNA sequence identification
       |__primer extension assay  1 rec.

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Relationships
Is a	partial RNA sequence identification
Part of
Synonyms & Secondary IDs
Synonyms
	"primer extension assay" EXACT PSI-MI-short
Secondary IDs

External Crossreferences & Linkouts