FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
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Term primer extension assay ID (Ontology) MI:2203 (Molecular Interactions)
Definition Primer extension can be used to determine the 3' end of a given sequence of RNA. This technique requires a radiolabelled primer which is complementary to a region near the 3' end of the RNA. The primer is allowed to anneal to the RNA and reverse transcriptase is used to synthesize a strand of DNA from the RNA until it reaches the 5' end of the RNA. By denaturing the hybrid and using the extended primer DNA strand as a marker on an electrophoretic gel, it is possible to determine the transcriptional start site.[ PubMed:23378648 ]
Also Known As "primer extension assay"
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 primer extension assay (all annotations which use CV term, excluding "NOT" statements)       1
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  partial nucleotide sequence identification
   |__partial RNA sequence identification
       |__primer extension assay  1 rec.
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Is a partial RNA sequence identification
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  • "primer extension assay" EXACT PSI-MI-short
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