FB2025_01 , released February 20, 2025
Allele: Dmel\Syt4UAS.cLa
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General Information
Symbol
Dmel\Syt4UAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Littleton
FlyBase ID
FBal0100393
Feature type
allele
Associated gene
Dmel\Syt4
Associated Insertion(s)
Carried in Construct
P{UAS-SytIV.L}
Also Known As
UAS-Syt4
Key Links
Transgenic product class
wild_type
(Littleton et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Littleton et al., 1999)
Mutagen
in vitro construct
(Littleton et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-SytIV.L}
Cytology
Description

A 3.1kb fragment encompassing the Syt4 ORF is driven by UASt sequences.

(Littleton et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Syt4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of SytIVScer\UAS.cLa under the control of Scer\GAL4elav.PLu in a wild-type background has no effect on excitatory junctional potential (EJP) amplitude, quantal content, miniature EJP (mEJP) frequency or mEJP amplitude at the neuromuscular junction.

      (Robinson et al., 2002)

      Overexpression of SytIVScer\UAS.cLa in neurons, driven by Scer\GAL4elav.PLu creates larvae that when tested electrophysiologically by stimulation of the neuromuscular junction, produce peak amplitudes of the excitatory junction potential that are significantly (about 20%) lower than controls. Continued stimulation showed no further depletion over time. No decrease in muscle miniature excitatory junction potential (MEJP) amplitude or muscle resting membrane potential is seen in SytIVScer\UAS.cLa,Scer\GAL4elav.PLu larvae, however MEJP frequency is decreased by about a third.

      (Littleton et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Overexpression of SytIVScer\UAS.cLa, under the regulation of both Scer\GAL4elav-C155 and Scer\GAL4elav.PLu does not rescue the sytAD4/sytN13 phenotype. However, this overexpression does result in a 96% reduction in the expected Mendelian ratios. Overexpression of SytIVScer\UAS.cLa, under the regulation of Scer\GAL4elav-C155, in a sytAD4/sytN13 null mutant background has no effect on the synaptic transmission defects. Only slow release reflecting the asynchronous component of fusion is observed with the evoked EJP unchanged from the null mutant alone.

      (Adolfsen et al., 2004)

      EJP amplitude at the neuromuscular junction is restored to near wild-type levels in sytAD4 homozygous larvae if they are also expressing SytIVScer\UAS.cLa under the control of Scer\GAL4elav.PLu. The increase in frequency of mEJPs is also rescued to wild-type in these larvae.

      (Robinson et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/Syt4UAS.cLa rescues Df(3R)rn16/Syt4BA1

      (Korkut et al., 2013)

      Scer\GAL4Mhc.PW/Syt4UAS.cLa rescues Df(3R)rn16/Syt4BA1

      (Korkut et al., 2013)

      Scer\GAL4Mhc.PW/Syt4UAS.cLa rescues Df(3R)rn16

      (Yoshihara et al., 2005)
      Fails to rescue

      Scer\GAL4elav-C155/Syt4UAS.cLa fails to rescue Df(3R)rn16

      (Yoshihara et al., 2005)
      Comments

      The lack of high-frequency-stimulation-induced miniature release (HFMR) seen in Df(3R)rn16 animals is rescued by expression of SytIVScer\UAS.cLa under the control of Scer\GAL4Mhc.PW (postsynaptic expression) but not by expression of SytIVScer\UAS.cLa under the control of Scer\GAL4elav-C155 (presynaptic expression).

      (Yoshihara et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Syt4Scer\UAS.cLa
      Syt4UAS.cLa
      SytIVScer\UAS.cLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Littleton
      (Littleton et al., 1999)
      Secondary FlyBase IDs
        References (6)