tarsal segment 1 & leg sensillum, with Scer\GAL4sca-109-68
The formation of the most anterior midgut constriction is abnormal in embryos expressing UbxIa.Scer\UAS.cRa under the control of Scer\GAL4how-24B, while the formation of the second and third midgut constrictions is normal.
Expression of UbxIa.Scer\UAS.R under the control of Scer\GAL4sca-109-68 does not result in loss of the apical bristle on the T2 leg. At 18oC, the preapical bristle on T2 is transformed towards the morphology of a T3 preapical bristle (the stout shaft morphology is transformed to a much finer shaft). The short bristles on the T2 basitarsus are transformed towards the long stout morphology of those normally seen on the T3 basitarsus. Expression of UbxIa.Scer\UAS.R under the control of Scer\GAL4dpp.blk1 results in loss of the apical bristle on the T2 leg in 45/50 cases. The preapical bristle is rarely suppressed in these flies (10/50 cases). The mechanosensory microchaetae on the dorsal T2 leg arise normally.
Clones in the wing expressing UbxIa.Scer\UAS.R under the control of Scer\GAL4Act5C.PI and induced at 0-4 hours after puparium formation show no obvious phenotype. Clones in the wing expressing UbxIa.Scer\UAS.R under the control of Scer\GAL4Act5C.PI and induced 48 hours before puparium formation are sometimes transformed to resemble haltere cells, but this transformation is not fully penetrant. Not all clones show any transformation, and even in those that do, not all cells are transformed. Many of the clones have fewer cells than control clones. Clones in the wing expressing UbxIa.Scer\UAS.R under the control of Scer\GAL4Act5C.PI and induced during embryogenesis give rise to large patches of tissue in the wing that differentiate as tissue very similar to the normal haltere.
H. Reed M. Akam