Allele Dmel\Gad1^Scer\UAS.cFa

General Information
Symbol	Dmel\Gad1^Scer\UAS.cFa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Featherstone	FlyBase ID	FBal0119480
Feature type	allele	Associated gene	Dmel\Gad1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Featherstone et al., 2000)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A 2160bp EcoRI fragment containing the Gad1 cDNA, including 186bp of 5' untranslated sequence, 1530bp of coding sequence, and 444bp 3' untranslated sequence. (Featherstone et al., 2000)
Carried in construct	P{UAS-gad.F} (Featherstone et al., 2002, Featherstone et al., 2000)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective, with Scer\GAL4^elav.PLu (Featherstone et al., 2002)
Phenotype Manifest In

Detailed Description
	Statement Reference The amplitude of glutamate-gated currents is increased in embryos expressing Gad1^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu compared to wild-type (2198 +/- 270 pA compared to 1691 +/- 71 pA), when glutamate is pressure-ejected onto the postsynaptic neuromuscular junction. Single-channel current amplitude of postsynaptic glutamate receptors is not different from wild type in the neuromuscular junction of embryos expressing Gad1^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu. The amplitude of spontaneous excitatory junctional currents is increased in the neuromuscular junction of embryos expressing Gad1^Scer\UAS.cFa under the control of Scer\GAL4^elav.PLu compared to wild type (213 +/- 52 pA compared to 141 +/- 14 pA). (Featherstone et al., 2002)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Gad1^Scer\UAS.cFa/Scer\GAL4^elav.PLu rescues Gad1^E143K (Featherstone et al., 2000) Gad1^Scer\UAS.cFa/Scer\GAL4^elav.PLu rescues Gad1^L352F (Featherstone et al., 2000)
Partially rescues	Scer\GAL4^eve.RKK/Gad1^Scer\UAS.cFa partially rescues Gad1^L352F (Featherstone et al., 2000)
Comments	The addition of Gad1^Scer\UAS.cFa driven by Scer\GAL4^elav.PLu to Gad1^E143K and Gad1^L352F homozygotes, rescues the lethality seen in these flies. 72% of Gad1^L352F rescued homozygotes, and 27% of Gad1^E143K rescued homozygotes are apparently wild-type. (Featherstone et al., 2000)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Gad1^Scer\UAS.cFa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Featherstone (Featherstone et al., 2000)
Secondary FlyBase IDs

References ( 2 )
Research paper	Featherstone et al., 2002, Nat. Neurosci. 5(2): 141--146 Developmental regulation of glutamate receptor field size by nonvesicular glutamate release. [FBrf0145170] Featherstone et al., 2000, Neuron 27(1): 71--84 Presynaptic glutamic acid decarboxylase is required for induction of the postsynaptic receptor field at a glutamatergic synapse. [FBrf0130303]

Allele Dmel\Gad1Scer\UAS.cFa

Allele Dmel\Gad1^Scer\UAS.cFa