FB2025_01 , released February 20, 2025
Allele: Dmel\Gad1UAS.cFa
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General Information
Symbol
Dmel\Gad1UAS.cFa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Featherstone
FlyBase ID
FBal0119480
Feature type
allele
Associated gene
Dmel\Gad1
Associated Insertion(s)
Carried in Construct
P{UAS-gad.F}
Key Links
Transgenic product class
cDNA
(Featherstone et al., 2000)
wild_type
(Featherstone et al., 2000)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Featherstone et al., 2000)
wild_type
(Featherstone et al., 2000)
Mutagen
in vitro construct
(Featherstone et al., 2000)
Progenitor genotype
Carried in construct
P{UAS-gad.F}
Cytology
Description

A 2160bp EcoRI fragment containing the Gad1 cDNA, including 186bp of 5' untranslated sequence, 1530bp of coding sequence, and 444bp 3' untranslated sequence.

(Featherstone et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Gad1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The amplitude of glutamate-gated currents is increased in embryos expressing Gad1Scer\UAS.cFa under the control of Scer\GAL4elav.PLu compared to wild-type (2198 +/- 270 pA compared to 1691 +/- 71 pA), when glutamate is pressure-ejected onto the postsynaptic neuromuscular junction. Single-channel current amplitude of postsynaptic glutamate receptors is not different from wild type in the neuromuscular junction of embryos expressing Gad1Scer\UAS.cFa under the control of Scer\GAL4elav.PLu. The amplitude of spontaneous excitatory junctional currents is increased in the neuromuscular junction of embryos expressing Gad1Scer\UAS.cFa under the control of Scer\GAL4elav.PLu compared to wild type (213 +/- 52 pA compared to 141 +/- 14 pA).

      (Featherstone et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Gad1UAS.cFa/Scer\GAL4elav.PLu rescues Gad1E143K

      (Featherstone et al., 2000)

      Gad1UAS.cFa/Scer\GAL4elav.PLu rescues Gad1L352F

      (Featherstone et al., 2000)
      Partially rescues

      Scer\GAL4eve.RKK/Gad1UAS.cFa partially rescues Gad1L352F

      (Featherstone et al., 2000)
      Comments

      The addition of Gad1Scer\UAS.cFa driven by Scer\GAL4elav.PLu to Gad1E143K and Gad1L352F homozygotes, rescues the lethality seen in these flies. 72% of Gad1L352F rescued homozygotes, and 27% of Gad1E143K rescued homozygotes are apparently wild-type.

      (Featherstone et al., 2000)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gad1Scer\UAS.cFa
      Gad1UAS.cFa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Featherstone
      (Featherstone et al., 2000)
      Secondary FlyBase IDs
        References (2)