FB2025_01 , released February 20, 2025
Allele: Dmel\Syt1KQ.UAS
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General Information
Symbol
Dmel\Syt1KQ.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0137074
Feature type
allele
Associated gene
Dmel\Syt1
Associated Insertion(s)
Carried in Construct
P{UAS-syt.KQ}
Key Links
Transgenic product class
missense_variant
(Mackler and Reist, 2001)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Mackler and Reist, 2001)
Mutagen
in vitro construct
(Mackler and Reist, 2001)
Progenitor genotype
Syt1UAS.cMa
(Mackler and Reist, 2001)
Carried in construct
P{UAS-syt.KQ}
Cytology
Description

Construct: Amino acid replacement: K379Q. Amino acid replacement: K380Q. Amino acid replacement: K384Q.

(Mackler and Reist, 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Syt1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      There is no difference in τ values in Syt1AD4/Syt1N13; Syt1KQ.Scer\UAS Scer\GAL4elav.PLu flies in response to a 50Hz, 10s stimulus, indicating that endocytosis rate is normal at Syt1KQ.Scer\UAS synapses.

      Syt1AD4/Syt1N13; Syt1KQ.Scer\UAS Scer\GAL4elav.PLu synapses at rest have a mean vesicle diameter equivalent to controls. Following stimulation, however, they show a significant further increase in mean vesicle diameter. Vesicles with diameters in excess of 80nm are observed.

      (Poskanzer et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Syt1KQ.UAS/Scer\GAL4elav.PLu partially rescues Syt1AD4

      (Mackler and Reist, 2001)
      Comments

      Expression of sytKQ.Scer\UAS under the control of Scer\GAL4elav.PLu partially rescues the lethality of sytAD4 flies. The rescued animals are distinctly uncoordinated; sytAD4 homozygous larvae which are also expressing sytKQ.Scer\UAS under the control of Scer\GAL4elav.PLu crawl sluggishly and move their mouth hooks at a slower frequency than normal. sytAD4 homozygous adults which are also expressing sytKQ.Scer\UAS under the control of Scer\GAL4elav.PLu show slow, uncoordinated and arrhythmic limb placement when climbing. The mean excitatory junction potential (EJP) amplitude at the neuromuscular junction of sytAD4 homozygous larvae which are also expressing sytKQ.Scer\UAS under the control of Scer\GAL4elav.PLu is significantly reduced compared to the mean EJP amplitude of sytAD4 homozygous larvae which are also expressing sytScer\UAS.cMa under the control of Scer\GAL4elav.PLu. The mean miniature EJP (mEJP) amplitude at the neuromuscular junction of sytAD4 homozygous larvae which are also expressing sytKQ.Scer\UAS under the control of Scer\GAL4elav.PLu is not significantly different from the mean mEJP amplitude of sytAD4 homozygous larvae which are also expressing sytScer\UAS.cMa under the control of Scer\GAL4elav.PLu. The mean mEJP frequency at the neuromuscular junction of sytAD4 homozygous larvae which are also expressing sytKQ.Scer\UAS under the control of Scer\GAL4elav.PLu is approximately twice that of sytAD4 homozygous larvae which are also expressing sytScer\UAS.cMa under the control of Scer\GAL4elav.PLu.

      (Mackler and Reist, 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Syt1KQ.Scer\UAS
      Syt1KQ.UAS
      sytKQ.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)