FlyBase Allele Report: Dmel\RpL10Ab[KG09231]

General Information

Symbol

Dmel\RpL10Ab^KG09231

Species

D. melanogaster

Name

FlyBase ID

FBal0148170

Feature type

allele

Associated gene

Dmel\RpL10Ab

Associated Insertion(s)

P{SUPor-P}RpL10Ab^KG09231

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}RpL10Ab^KG09231

Cytology

Description

P{SUPor-P}RpL10Ab^KG09231 is inserted -28bp upstream of the RpL10Ab transcriptional start site.

(Wonglapsuwan et al., 2011)

Allele components

Component

Use(s)

Inserted element

P{SUPor-P}

insertional mutagenesis tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:11,822,824..11,822,824 [-]

(Wonglapsuwan et al., 2011, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage

(Wonglapsuwan et al., 2011)

Phenotype Manifest In

egg chamber | germline clone

(Wonglapsuwan et al., 2011)

follicle cell | germline clone

(Wonglapsuwan et al., 2011)

nucleolus | germline clone

(Wonglapsuwan et al., 2011)

nucleus | germline clone

(Wonglapsuwan et al., 2011)

Detailed Description

Statement

Reference

RpL10Ab^KG09231 germline clones fail to progress into mid-oogenesis. Approximately 50% of dying egg chambers exhibit a loss of follicle cells around the egg chamber, while the nurse cells appear normal. Nuclei of RpL10Ab^KG09231 germline follicle cells display abnormal morphology and are much larger than heterozygous nuclei. Moreover, the nucleoli of these clones are larger and more prominent compared to controls. RpL10Ab^KG09231 germline clones are larger and reduced in number compared to controls, suggesting a defect in proliferation or survival. Only 23% of RpL10Ab^KG09231 stage 10 egg chambers exhibit follicle cell clones.

(Wonglapsuwan et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RpL10Ab^KG09231 has lethal - all die before end of larval stage phenotype, suppressible | partially by Fmer\RpL10A^UAS.cWa/Scer\GAL4^Act5C.PI

(Wonglapsuwan et al., 2011)

RpL10Ab^KG09231 has lethal - all die before end of larval stage phenotype, suppressible | partially by Fmer\RpL10A^UAS.cWa/Scer\GAL4^arm.PS

(Wonglapsuwan et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Ubiquitous expression of Zzzz\RpL10A^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI, leads to 16% viability of RpL10Ab^KG09231 flies whereas expression under the control of Scer\GAL4^arm.PS leads to 38% viability in RpL10Ab^KG09231 mutants. In addition, these rescued flies are fertile, indicating that Zzzz\RpL10A^Scer\UAS.cWa has functional conservation with RpL10Ab.