FB2025_01 , released February 20, 2025
Allele: Dmel\BsgNP6293
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General Information
Symbol
Dmel\BsgNP6293
Species
D. melanogaster
Name
FlyBase ID
FBal0155253
Feature type
allele
Associated gene
Dmel\Bsg
Associated Insertion(s)
P{GawB}BsgNP6293
Carried in Construct
Also Known As
NP6293-Gal4, NP6293, PG-Gal4
Key Links
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    P{GawB}BsgNP6293
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    P{GawB}
    Encoded product / tool
    GAL4
    Mutations Mapped to the Genome
    Curation Data
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        BsgNP6293/Df(2L)Exel7034 hemizygous flies are semi-lethal, with approximately 30% reaching third larval instar.

        Axonal targeting is not altered to a visible degree in BsgNP6293/Df(2L)Exel7034 transheterozygotes. The proportion of very large boutons is greatly increased compared to controls. The observed increase in bouton size is associated with a concomitant reduction of both NMJ branching and bouton number, keeping the overall NMJ size close to normal. Active zones are found at normal frequency and exhibit characteristic electron-dense specializations. The organisation of the presynaptic F-actin network is altered in BsgNP6293/Df(2L)Exel7034 NMJs.

        Preferential association of vesicles with the bouton cortex is lost in approximately 60% of NMJ branches in BsgNP6293/Df(2L)Exel7034 larvae.

        The amplitude of the postsynaptic response to the fusion of single vesicles is increased above wild-type levels in BsgNP6293/Df(2L)Exel7034 mutants. This effect is most likely related to the observed enlargement of the postsynaptic glutamate receptor clusters, given that no increase in the size of synaptic vesicles is found in BsgNP6293/Df(2L)Exel7034 mutants compared with controls. Notably, the frequency of spontaneous release events is strongly elevated in BsgNP6293/Df(2L)Exel7034 mutants. The mean amplitude of nerve-evoked excitatory junctional currents (eEJCs) is also increased at BsgNP6293/Df(2L)Exel7034 NMJs.

        The temporal profile of BsgNP6293/Df(2L)Exel7034 mutant eEJCs is lengthened, reflecting a dramatic and atypically delayed release of vesicles. Although the charge carried by BsgNP6293/Df(2L)Exel7034 mutant single vesicles is only moderately increased (1.5-fold), a near eightfold elevation of the charge transferred to the postsynapse after exocytosis occurs upon initial nerve stimulation.

        (Besse et al., 2007)

        Homozygous mutants show no defects in germ band retraction and dorsal closure.

        (Reed et al., 2004)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Enhancer of
        Statement
        Reference

        BsgNP6293/Bsg[+] is an enhancer of embryo | dorsal closure stage phenotype of mys1

        (Reed et al., 2004)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Homozygous mys1 embryos also heterozygous for BsgNP6293 show an enhancement of the dorsal hole phenotype seen in mys1 embryos.

        (Reed et al., 2004)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments

        Presynaptic expression of BsgScer\UAS.cBa (under the control of Scer\GAL4elav-C155 in a BsgNP6293/Df(2L)Exel7034 mutant background rescues both the asynchronous evoked release and the high frequency of spontaneous release. Presynaptic expression decreases the amplitude of eEJCs and the frequency of single vesicle release below control levels, indicating a dose-dependent role. Expression post-synaptically, under the control of Scer\GAL4Mhc.PW does not rescue.

        Expression of wild-type BsgScer\UAS.cBa solely in muscles (using Scer\GAL4Mhc.PW or Scer\GAL4how-24B) or in neurons (using Scer\GAL4elav-C155) partially, but significantly, rescued both the increase in bouton size and the reduction of bouton number observed in BsgNP6293/Df(2L)Exel7034 mutant larvae. Near-complete rescue of bouton size and junction growth is obtained upon expression of BsgScer\UAS.cBa both pre- and postsynaptically (using Scer\GAL4Mhc.PW or Scer\GAL4how-24B post-synaptically and Scer\GAL4elav-C155 pre-synaptically).

        (Besse et al., 2007)
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (4)
        Reported As
        Symbol Synonym
        Bsg6293
        (Besse et al., 2007)
        BsgNP6293
        bsgNP6293
        (Reed et al., 2004)
        gelNP6293-5-1
        Name Synonyms
        Secondary FlyBase IDs
          References (2)