Allele Dmel\CrebB^cor.Scer\UAS

General Information
Symbol	Dmel\CrebB^cor.Scer\UAS	Species	D. melanogaster
Name	corrected	FlyBase ID	FBal0184295
Feature type	allele	Associated gene	Dmel\CrebB

Allele class
Mutagen	in vitro construct - coding region fusion
Recent Updates
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FB2013_03	Genes CrebB
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Perazzona et al., 2004)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	CrebB^mut.Scer\UAS (Perazzona et al., 2004)
Nature of the lesion	Statement Reference Construct: Coding region for CrebB-17A is corrected to wild type sequence by replacement of first 139bp of the mutated CrebB-17A cassette in CrebB-17A^mut.Scer\UAS with wild type sequences from the CrebB-17A isoform b. Expression is governed by Scer\UAS regulatory sequences. (Perazzona et al., 2004)
Carried in construct	P{UAS-CrebB-17A-a.cor} (Perazzona et al., 2004, Chen and Ganetzky, 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| heat sensitive, with Scer\GAL4^hs.PB (Perazzona et al., 2004) memory defective, with Scer\GAL4^hs.PB (Perazzona et al., 2004) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^elav.PLu (Chen and Ganetzky, 2012)
Phenotype Manifest In
	embryonic/larval neuromuscular junction \| third instar larval stage, with Scer\GAL4^elav.PLu (Chen and Ganetzky, 2012) NMJ bouton \| third instar larval stage, with Scer\GAL4^elav.PLu (Chen and Ganetzky, 2012)
Detailed Description
	Statement Reference Overexpression of CrebB-17A[cor.Scer\UAS] driven by Scer\GAL4[elav.PLu] causes a mild, but significant, increase in synaptic bouton numbers at larval neuromuscular junctions. (Chen and Ganetzky, 2012) Heat shock induced expression of CrebB-17A^cor.Scer\UAS causes a slow lethality. Four days after 30 min heat shock at 37^oC over 50% of the flies were dead, with lethality reaching 99% by day 7. Without heat shock, though in combination Scer\GAL4^hs.PB with which nevertheless increases expression of CrebB-17A^cor.Scer\UAS, the positive effect of 5x spaced training in the olfactory classical conditioning LTM assay (tested at 4 days) is abolished. Thus expression depresses learning in this assay. (Perazzona et al., 2004)
External Data
Linkouts
Interactions
Phenotypic Class
NOT suppressed by
Statement Reference CrebB^cor.Scer\UAS has neuroanatomy defective \| third instar larval stage phenotype, non-suppressible by Df(1)Exel9051/Df(1)Exel9051 (Chen and Ganetzky, 2012)
Phenotype Manifest In
NOT suppressed by
Statement Reference CrebB^cor.Scer\UAS has embryonic/larval neuromuscular junction \| third instar larval stage phenotype, non-suppressible by Df(1)Exel9051/Df(1)Exel9051 (Chen and Ganetzky, 2012)
Additional Comments
Genetic Interactions
Statement Reference Overexpression of CrebB-17A[cor.Scer\UAS] under the control of Scer\GAL4[elav.PLu] in a Df(1)Exel9051/Y genetic background results in a mild neuromuscular junction (NMJ) overgrowth phenotype. (Chen and Ganetzky, 2012)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	9232 P{UAS-CrebB-17A-a.cor}T7.1, ry⁵⁰⁶ 9233 P{UAS-CrebB-17A-a.cor}T25.4, ry⁵⁰⁶
Notes on Origin
Discoverer

Comments
	Full length CrebB-17A product is encoded by the allele. (Perazzona et al., 2004)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CrebB-17A^cor.Scer\UAS CrebB^cor.Scer\UAS
Name Synonym	corrected (Perazzona et al., 2004)
Secondary FlyBase IDs

References ( 2 )
Research paper	Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543 A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500] Perazzona et al., 2004, J. Neurosci. 24(40): 8823--8828 The role of cAMP response element-binding protein in Drosophila long-term memory. [FBrf0180428]

Allele Dmel\CrebBcor.Scer\UAS

Allele Dmel\CrebB^cor.Scer\UAS