FlyBase Allele Report: Dmel\Tdc2[RO54]

General Information

Symbol

Dmel\Tdc2^RO54

Species

D. melanogaster

Name

FlyBase ID

FBal0189582

Feature type

allele

Associated gene

Dmel\Tdc2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Cole et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: E51K. Point mutation in highly conserved pyridoxal-dependent decarboxylase conserved domain.

(Cole et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:6,688,290..6,688,290 [-]

Nucleotide change:

G6688290A

Amino acid change:

E51K | Tdc2-PA

Reported amino acid change:

E51K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Cole et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain

(Cole et al., 2005)

egg chamber (with Df(2R)42)

(Deady and Sun, 2015)

embryonic/larval neuromuscular junction | third instar larval stage

(Koon et al., 2011)

multidendritic neuron

(Koon et al., 2011)

type II bouton

(Koon et al., 2011)

Detailed Description

Statement

Reference

Tdc2^RO54/Df(2R)42 mutant adult females display defects in trimming of posterior follicle cells and produce fewer trimmed eggs.

(Deady and Sun, 2015)

Virgin Tdc2^RO54 mutant females show increased receptivity compared to wild type virgin females. Mated Tdc2^RO54 females lay very few eggs and remain highly receptive after copulation, displaying low levels of rejection behaviors such as ovipositor extrusion compared with mated controls. Wild type males court Tdc2^RO54 mated females significantly more than wild type mated females.

(Rezával et al., 2014)

Mutants show decreased activity and decreased circadian rhythmicity.

(Huang et al., 2013)

Tbh^nM18/+ larvae have a significant reduction in the distance they crawl, relative to controls; locomotion tends to be further reduced in homozygotes.

(Selcho et al., 2012)

Tdc2^RO54 mutant third instar larvae exhibit an increase in the number of synaptopods (dynamic filopodia-like extensions).

Tdc2^RO54 mutant neuromuscular junctions exhibit a wild-type response to the addition of 10υM octopamine with an increase in the number of synaptopods.

Tdc2^RO54 mutant third instar larval neuromuscular junctions exhibit a decrease in the number of type II boutons and terminal branches.

Tdc2^RO54 mutant third instar larvae exhibit a 15% increase in locomotor speed in response to starvation for 2 hours, compared to a 36% increase in wild-type.

(Koon et al., 2011)

Tdc2^RO54 male adult flies show reduced locomotor activity compared to control flies; this effect is much greater in homozygotes but can still be observed in heterozygotes.

Tdc2^RO54 homozygous adult flies are hypersensitive to cocaine; these flies show at least a 5X increase in responsiveness relative to controls although the types of behavior induced are indistinguishable from controls. Tdc2^RO54 heterozygotes also show significant cocaine sensitivity but this is less severe than homozygotes. The Tdc2^RO54 flies show similar behavioral sensitization to repeated doses of cocaine to control flies.

(Hardie et al., 2007)

Tdc2^RO54 females fail to lay eggs. As mature eggs and egg remnants can be found in the calyx, lateral and common oviduct, it appears that these mutants achieve ovulation but not oviposition. This phenotype can be partially rescued by feeding with tyramine and octopamine. Tdc2^RO54/Df(2R)42 transheterozygotes also fail to lay eggs. Tdc2^RO54 flies have reduced levels of tyramine and octopamine in the brain compared to wild type.

(Cole et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Tdc2^RO54 has abnormal chemosensory behavior | semidominant | adult stage phenotype, suppressible by Scer\GAL4^Tdc2.PC/Tdc1^UAS.cCa

(Hardie et al., 2007)

Tdc2^RO54 has female sterile | recessive phenotype, suppressible by Scer\GAL4^Tdc2.PC/Tdc1^UAS.cCa

(Cole et al., 2005)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Tdc2^RO54 has adult brain phenotype, non-suppressible by Scer\GAL4^Tdc2.PC/Tdc1^UAS.cCa

(Cole et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tdc1^Scer\UAS.cCa under the control of Scer\GAL4^Tdc2.PC results in complete suppression of the cocaine sensitivity phenotype of Tdc2^RO54 flies.

(Hardie et al., 2007)

Expression of Tdc1^Scer\UAS.cCa, under the control of Scer\GAL4^Tdc2.PC, completely rescues the egg retention phenotype of Tdc2^RO54 mutants. However, expression of this transgene fails to rescue the reduced neural tyramine and octopamine phenotype.

(Cole et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Tdc2^RO54 is partially rescued by Tdc2^UAS.cCb/Scer\GAL4^Tdc2.PC

(Cole et al., 2005)

Not rescued by

Tdc2^RO54 is not rescued by Tdc2^UAS.cCb/Scer\GAL4^Tdc2.PC

(Hardie et al., 2007)

Comments

Expression of Tdc2^Scer\UAS.cCb, under the control of Scer\GAL4^Tdc2.PC, partially rescues the egg retention phenotype of Tdc2^RO54 mutants. This expression also increases levels of tyramine and octopamine in the brains of Tdc2^RO54 flies.

(Cole et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

94000

w¹¹¹⁸; Tdc2^RO54 cn¹ bw¹/CyO

Notes on Origin

Discoverer

T. Schupbach

(Cole et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tdc2^RO54

(Ma and Freeman, 2020, Huang et al., 2016, Deady and Sun, 2015, Rezával et al., 2014, Chen et al., 2013, Pizzo et al., 2013, Selcho et al., 2012, Koon et al., 2011, Crocker and Sehgal, 2008, Hardie et al., 2007)

tdc2^R054

(Li et al., 2021)

tdc2^RO54

(Kim et al., 2021, Ma et al., 2016, Huang et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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