FlyBase Allele Report: Dmel\wnd[1]

General Information

Symbol

Dmel\wnd¹

Species

D. melanogaster

Name

FlyBase ID

FBal0194609

Feature type

allele

Associated gene

Dmel\wnd

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Collins et al., 2006)

Progenitor genotype

Cytology

Description

wnd¹ habours a mutation in a conserved residue in the kinase domain.

(Collins et al., 2006)

Amino acid replacement: G172E.

(Collins et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:19,629,385..19,629,385 [-]

Nucleotide change:

G19629385A

Amino acid change:

G173E | wnd-PA; G173E | wnd-PB; G173E | wnd-PC; G146E | wnd-PD

Reported amino acid change:

G172E

Comment:

The mutation was mapped to G173 because there is no G at position 172 in the reference sequence. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Collins et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X syndrome

modeled by Fmr1^Δ50M

(Russo and DiAntonio, 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | conditional (with wnd²)

(Xiong et al., 2010)

abnormal neuroanatomy | larval stage (with Df(3L)ED229)

(Klinedinst et al., 2013)

abnormal neuroanatomy | larval stage (with wnd²)

(Klinedinst et al., 2013)

Phenotype Manifest In

larval segmental nerve | conditional (with wnd²)

(Xiong et al., 2010)

NMJ bouton | larval stage (with Df(3L)ED229)

(Klinedinst et al., 2013)

NMJ bouton | larval stage (with wnd²)

(Klinedinst et al., 2013)

Detailed Description

Statement

Reference

wnd¹/wnd³ transheterozygotes do not show grooming defects, nor any obvious adult mushroom body defects.

(Russo and DiAntonio, 2019)

wnd¹/wnd³ animals show severely reduced axon outgrowth in ddaC neurons after axon severing, while dendrite regeneration after complete removal of the dendrites appears normal.

(Stone et al., 2014)

wnd¹/Df(3L)ED229 larvae show defects in axonal transport, resulting in accumulations of protein in nerves.

wnd¹/wnd² and wnd¹/Df(3L)ED229 larvae show an increase in maximum bouton diameter at the neuromuscular junction compared to controls.

The ability of injured axons to sprout after injury is inhibited in wnd¹/wnd² larvae.

(Klinedinst et al., 2013)

Axonal sprouting of the injured axon after a crush injury to the segmental nerve in third instar larvae is dramatically inhibited in wnd¹/wnd² animals.

(Xiong et al., 2010)

wnd¹ mutants exhibit wild-type synapse size.

(Franciscovich et al., 2008)

wnd¹ mutants show no reduction in synaptic growth.

wnd¹/wnd² mutants exhibit normal synaptic function.

(Collins et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

wnd³/wnd¹ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Russo and DiAntonio, 2019)

wnd³/wnd¹ is a suppressor of abnormal neuroanatomy | adult stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Russo and DiAntonio, 2019)

wnd³/wnd¹ is a suppressor of abnormal size | adult stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Russo and DiAntonio, 2019)

wnd³/wnd¹ is a suppressor of abnormal grooming behavior | progressive phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Russo and DiAntonio, 2019)

wnd¹/wnd² is a suppressor | partially of lethal | dominant phenotype of Scer\GAL4^elav.PU, SkpA^GD65, faf^UAS.cUa

(Brace et al., 2014)

wnd¹/wnd² is a suppressor | partially of abnormal neurophysiology | larval stage phenotype of SkpA^GD65

(Brace et al., 2014)

wnd[+]/wnd¹ is a suppressor | partially of abnormal neuroanatomy phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

wnd[+]/wnd¹ is a suppressor | partially of increased cell growth rate phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

wnd¹ is a suppressor of abnormal neuroanatomy phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

wnd¹ is a suppressor of increased cell growth rate phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

wnd[+]/wnd¹ is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, sgg^A81T.UAS

(Franciscovich et al., 2008)

wnd¹/wnd² is a suppressor of abnormal neurophysiology phenotype of hiw^ND8

(Collins et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

wnd³/wnd¹ is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Russo and DiAntonio, 2019)

wnd³/wnd¹ is a suppressor of adult mushroom body alpha-lobe phenotype of Fmr1^Δ50M/Df(3R)Exel6265

(Russo and DiAntonio, 2019)

wnd¹/wnd² is a suppressor | partially of NMJ bouton | maternal effect | larval stage phenotype of SkpA^GD65

(Brace et al., 2014)

wnd¹/wnd² is a suppressor | partially of embryonic/larval neuromuscular junction | maternal effect | larval stage phenotype of SkpA^GD65

(Brace et al., 2014)

wnd¹ is a suppressor of NMJ bouton phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

wnd¹ is a suppressor of neuromuscular junction phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

wnd[+]/wnd¹ is a suppressor | partially of NMJ bouton phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

wnd[+]/wnd¹ is a suppressor | partially of neuromuscular junction phenotype of Atg1^UAS.cSa, Scer\GAL4^elav.PLu

(Shen and Ganetzky, 2009)

wnd[+]/wnd¹ is a suppressor of synapse phenotype of Scer\GAL4^elav-C155, sgg^A81T.UAS

(Franciscovich et al., 2008)

wnd¹/wnd² is a suppressor of neuromuscular junction phenotype of Scer\GAL4^elav-C155, faf^EP381

(Collins et al., 2006)

wnd¹/wnd² is a suppressor of bouton phenotype of Scer\GAL4^elav-C155, faf^EP381

(Collins et al., 2006)

wnd[+]/wnd¹ is a suppressor of neuromuscular junction phenotype of hiw^ND8

(Collins et al., 2006)

wnd[+]/wnd¹ is a suppressor of bouton phenotype of hiw^ND8

(Collins et al., 2006)

wnd¹/wnd² is a suppressor of neuromuscular junction phenotype of hiw^ND8

(Collins et al., 2006)

wnd¹/wnd² is a suppressor of bouton phenotype of hiw^ND8

(Collins et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The synaptic overgrowth phenotype (increase in bouton number) seen at the neuromuscular junction in larvae derived from homozygous SkpA^GD65 females is significantly (but not completely) suppressed by wnd¹/wnd².

(Brace et al., 2014)

A wnd¹ heterozygous background partially suppresses the high levels of autophagy and bouton number found upon expression of Atg1^Scer\UAS.cSa under the control of Scer\GAL4^elav.PLu. A wnd¹ homozygous background completely suppresses the neuromuscular junction overgrowth phenotype.

(Shen and Ganetzky, 2009)

A wnd¹/+ background completely abolishes the synapse growth seen through sgg^{A81T.Scer\UAS} expression pan-neuronally under the control of Scer\GAL4^elav-C155.

(Franciscovich et al., 2008)

Removing one copy of wnd¹ suppresses the hiw^ND8 neuromuscular junction of muscle 4 phenotype by approximately 50%.

hiw^ND8; wnd¹/wnd² mutants are not significantly different from wild-type, indicating complete suppression of hiw^ND8.

wnd¹/wnd² mutants do not suppress the hiw^ND8 defect in quantal size: both the amplitude and the frequency of spontaneous miniature events in the hiw^ND8; wnd¹/wnd² double mutant are similar to wild-type. In contrast, the evoked potentials of the double mutant are only modestly increased, and this is due entirely to the increased quantal size. The quantal content, calculated as the excitatory junction potential (EJP) amplitude divided by the miniature EJP (mEJP) remains the same between hiw^ND8 and hiw^ND8; wnd¹/wnd² double mutants. Therefore wnd does not suppress the primary defect in hiw^ND8 synaptic function, the reduced number of vesicles released by the nerve.

(Collins et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51641

wnd¹ e¹/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Wnd-1

(Russo and DiAntonio, 2019)

wnd¹

(Wolfstetter et al., 2020, Akbergenova and Littleton, 2017, Li et al., 2017, Chen et al., 2016, Brace et al., 2014, Stone et al., 2014, Klinedinst et al., 2013, Nechipurenko and Broihier, 2012, Shin and Diantonio, 2011, Xiong et al., 2010, Shen and Ganetzky, 2009, Franciscovich et al., 2008, Wu et al., 2007, Collins et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (15)

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