FlyBase Allele Report: Dmel\tbc[JF03204]

General Information

Symbol

Dmel\tbc^JF03204

Species

D. melanogaster

Name

FlyBase ID

FBal0239702

Feature type

allele

Associated gene

Dmel\tbc

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03204}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03204}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03204

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

Detailed Description

Statement

Reference

The expression of tbc^JF03204 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including intracellular matrix material, as shown by the retention of both secr.GFP and Vkg.GFP.

(Ke et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^{SPARC-MI00329-GAL4}, tbc^JF03204 has embryonic/larval fat body | third instar larval stage phenotype, non-suppressible by Rab26^UAS.YFP, Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

Scer\GAL4^{SPARC-MI00329-GAL4}, tbc^JF03204 has extracellular matrix | third instar larval stage phenotype, non-suppressible by Rab26^UAS.YFP, Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

Other

Statement

Reference

Rab26^{T204N.UAS.YFP}, Scer\GAL4^Cg.PA, tbc^JF03204 has embryonic/larval fat body | third instar larval stage phenotype

(Ke et al., 2018)

Rab26^{T204N.UAS.YFP}, Scer\GAL4^Cg.PA, tbc^JF03204 has extracellular matrix | third instar larval stage phenotype

(Ke et al., 2018)

RabX6^UASp.YFP, Scer\GAL4^Cg.PA, tbc^JF03204 has embryonic/larval fat body | third instar larval stage phenotype

(Ke et al., 2018)

RabX6^UASp.YFP, Scer\GAL4^Cg.PA, tbc^JF03204 has extracellular matrix | third instar larval stage phenotype

(Ke et al., 2018)

Rab26^JF01684, Scer\GAL4^Cg.PA, tbc^JF03204 has embryonic/larval fat body | third instar larval stage phenotype

(Ke et al., 2018)

Rab26^JF01684, Scer\GAL4^Cg.PA, tbc^JF03204 has extracellular matrix | third instar larval stage phenotype

(Ke et al., 2018)

RabX6^{M69L.UASp.YFP}, Scer\GAL4^Cg.PA, tbc^JF03204 has embryonic/larval fat body | third instar larval stage phenotype

(Ke et al., 2018)

RabX6^{M69L.UASp.YFP}, Scer\GAL4^Cg.PA, tbc^JF03204 has extracellular matrix | third instar larval stage phenotype

(Ke et al., 2018)

RabX6^S22N.UAS.YFP, Scer\GAL4^Cg.PA, tbc^JF03204 has embryonic/larval fat body | third instar larval stage phenotype

(Ke et al., 2018)

RabX6^S22N.UAS.YFP, Scer\GAL4^Cg.PA, tbc^JF03204 has extracellular matrix | third instar larval stage phenotype

(Ke et al., 2018)

RabX6^JF02050, Scer\GAL4^Cg.PA, tbc^JF03204 has embryonic/larval fat body | third instar larval stage phenotype

(Ke et al., 2018)

RabX6^JF02050, Scer\GAL4^Cg.PA, tbc^JF03204 has extracellular matrix | third instar larval stage phenotype

(Ke et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of tbc^JF03204 and Rab26^{T204N.UAS.YFP} under the control of Scer\GAL4^Cg.PA (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion of intracellular matrix material, as shown by the retention of Vkg.GFP.

The co-expression of tbc^JF03204 and Rab26^JF01684 under the control of Scer\GAL4^Cg.PA (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion of intracellular matrix material, as shown by the retention of Vkg.GFP.

The co-expression of tbc^JF03204 and RabX6^{M69L.UASp.YFP} under the control of Scer\GAL4^Cg.PA (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion of intracellular matrix material, as shown by the retention of Vkg.GFP.

The co-expression of tbc^JF03204 and RabX6^S22N.UAS.YFP under the control of Scer\GAL4^Cg.PA (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion of intracellular matrix material, as shown by the retention of Vkg.GFP.

The co-expression of tbc^JF03204 and RabX6^JF02050 under the control of Scer\GAL4^Cg.PA (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion of intracellular matrix material, as shown by the retention of Vkg.GFP.

The co-expression of tbc^JF03204 and RabX6^UASp.YFP under the control of Scer\GAL4^Cg.PA (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion of intracellular matrix material, as shown by the retention of Vkg.GFP.

(Ke et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28776

y¹ v¹; P{TRiP.JF03204}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG32506^JF03204

tbc^JF03204

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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