UAS regulatory sequences drive expression of an inverted repeat.
The expression of RepJF02882 under the control of Scer\GAL4SPARC-MI00329-GAL4 (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including intracellular matrix material, as shown by the retention of secr.GFP, VSVG.GFP, Trol.GFP and Vkg.GFP. Expression under the control of Scer\GAL4Cg.PA leads to smaller endoplasmic reticulum exit sites (Sec16.GFP) that are uncoupled from the cis-Golgi (Grasp65.RFP).