The adulthood-only expression of putJF02664
under the control of Scer\GAL4esg.PU
(in combination with Gal80[ts], for temporal regulation of expression) leads to a significant decrease in the relative and absolute size of secondary cell nuclei compared to neighboring main cells and wild-type controls; this expression does not affect the size of main cell nuclei compared to controls. The adulthood-only expression under the control of Scer\GAL4dsx.KI
(and Gal80[ts]) leads to a significant decrease in the number of secretory vesicles within accessory gland secondary cells, but does not significantly affect the size of the largest multivesicular body/lysosome, as compared to controls; there is also a severe decrease in the secretion of (CD63-positive) exosomes into the accessory gland lumen.