Allele Dmel\KCNQ¹⁸⁶

General Information
Symbol	Dmel\KCNQ¹⁸⁶	Species	D. melanogaster
Name		FlyBase ID	FBal0244108
Feature type	allele	Associated gene	Dmel\KCNQ

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Ocorr et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EP}KCNQ^EP2074 (Ocorr et al., 2007)
Nature of the lesion	Statement Reference Imprecise excision of the progenitor insertion, deleting all transmembrane domains including the potassium selective pore region of the KCNQ channel. (Ocorr et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	developmental rate defective (Ocorr et al., 2007) fertile (Ocorr et al., 2007) short lived \| female (Ocorr et al., 2007) viable (Ocorr et al., 2007)
Phenotype Manifest In
	adult heart (Ocorr et al., 2007) adult heart \| conditional (Ocorr et al., 2007) adult heart muscle \| conditional (Ocorr et al., 2007)
Detailed Description
	Statement Reference KCNQ[186] mutant larvae take 1-2 days longer to develop, a reduced number of animals eclose, and the mean lifespan of females is reduced by 20-30%. Mutant adults show a drastically increased incidence of pacing-induced cardiac dysfunction compared to age-matched controls, and the elevated failure rates observed in young mutant flies does not increase further with age. Semi-intact KCNQ[186] fly heart preparations from young (1-3 week old) flies exhibit severely nonrhythmic beating patterns, and the incidence of arrhythmia increases more rapidly with age compared to control preparations. A dramatic age-dependent increase of the heart period (defined as the length of time between the ends of two consecutive diastolic intervals) compared with age-matched controls is also observed, and both diastolic intervals and systolic intervals show age-dependent increases in length. Electrophysiology field potential recordings from semi-intact KCNQ[186] fly heart preparations show that negative deflections do not immediately follow the initial positive potentials, and usually occur with significant delay compared to wildtype preparations. Semi-intact KCNQ[186] fly heart preparations show lower rates of muscle contractions compared to controls. In response to electrical stimulation, hearts exhibit fibrillation, markedly elevated diastolic tension, and extremely delayed recovery to baseline diastolic tension relative to wildtype hearts. (Ocorr et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	KCNQ¹⁸⁶ is rescued by KCNQ^Scer\UAS.cOa/Scer\GAL4^how-24B/Scer\GAL4^how-24B (Ocorr et al., 2007)
Comments	Expression of KCNQ[Scer\UAS.cOa] under the control of Scer\GAL4[how-24B] in a KCNQ[186] mutant background rescues the increased pacer-induced cardiac dysfunction rates and alterations in rhythmicity to levels comparable to those observed in control animals. (Ocorr et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	KCNQ¹⁸⁶ (Ocorr et al., 2007, Fabrizio et al., 2012)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Fabrizio et al., 2012, Fly 6(4): 261--272 mulet (mlt) encodes a tubulin-binding cofactor E-like homolog required for spermatid individualization in Drosophila melanogaster. [FBrf0220242] Ocorr et al., 2007, Proc. Natl. Acad. Sci. U.S.A. 104(10): 3943--3948 KCNQ potassium channel mutations cause cardiac arrhythmias in Drosophila that mimic the effects of aging. [FBrf0193190]

Allele Dmel\KCNQ186

Allele Dmel\KCNQ¹⁸⁶