Expression of sns^KK108577 under the control of Scer\GAL4^Ugt36A1.PK leads to 3D structural alterations in larval pericardial nephrocytes in the form of irregular (in width and shape) foot processes, reduced slit-like spaces and simpler labyrinths when compared to controls.

Adult flies expressing sns^KK108577 under the control of Scer\GAL4^Dot.PK display nephrocyte functional defects (measured by uptake assays) and reduced adult lifespan relative to controls.

Scer\GAL4^Dot.PK-mediated expression of sns^KK108577 completely abolishes the normal accumulation of ANF-RFP (expressed via Rnor\ANF^{Mhc.T:Disc\RFP}) in pericardial nephrocytes. EM analyses of mutant pericardial nephrocytes reveals reduced numbers of endocytic vesicles, shortened lacuna structures, and abolished nephrocyte diaphragms.

Adult males expressing sns^KK108577 under the control of Scer\GAL4^C855a show no defects in movement and are fertile. A scattered distribution of single muscle filaments are seen overlying the regular muscle sheath of the testis, in contrast to wild type.

Expression of sns^KK108577 in wing margin cells under the control of Scer\GAL4^wg-IS650 does not affect wing hair alignment. However, expression in hair cells under the control of Scer\GAL4^{neur-GAL4-A101} disrupts the normal hair alignment.