FlyBase Allele Report: Dmel\N[RNAi.UAS.cUa]

General Information

Symbol

Dmel\N^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0261122

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-N.RNAi.U}

Also Known As

UAS-Notch-RNAi, UAS-Notch^RNAi, notch^RNAi, UAS-N^RNAi, UAS-Notch RNAi

Key Links

Transgenic product class

RNAi_reagent

(Kuzina et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kuzina et al., 2011)

Mutagen

in vitro construct

(Kuzina et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-N.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of a N RNAi construct.

(Kuzina et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intestinal cancer

CEA

(Ayyaz et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intestinal cancer

is ameliorated by Smox^JF02320

(Ayyaz et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with N^l1N-ts1, Scer\GAL4^repo-M1B

(Kuzina et al., 2011)

decreased cell number | wandering third instar larval stage, with Scer\GAL4^btl.PS

(Pitsouli and Perrimon, 2013)

increased cell number | third instar larval stage, with Scer\GAL4^ap-md544

(Matsumoto et al., 2016)

neoplasia | adult stage, with Scer\GAL4^NP5130

(Yin and Xi, 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-only expression of N^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP5130 (using Gal80[ts] for the temporal control of expression) leads to abnormal intestinal stem cell-like and enteroendocrine cell-like tumors in the adult midgut.

(Yin and Xi, 2018)

The expression of N^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^ap-md544 leads to third instar larval wing discs exhibiting increased number of supraalar neurons, as compared to controls.

(Matsumoto et al., 2016)

Expression of N^{dsRNA.UAS.cUa} under the control of Scer\GAL4^btl.PS (in combination with a Gal80[ts] transgene to overcome embryonic and larval lethality) in spiracular branch (SB) growth zones 1 and 2, leads to a reduction of number of SB tracheoblasts.

(Pitsouli and Perrimon, 2013)

Expression of N^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^repo-M1B enhances the N^l1N-ts1 early axon phenotype.

(Kuzina et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has neoplasia | adult stage phenotype, suppressible | partially by phyl^GD12579, Scer\GAL4^NP5130

(Yin and Xi, 2018)

Other

Statement

Reference

Dronc^{KO.-FRT.SunTag(Unk),Tag:HA,mCherry}/Dronc^KO, N^RNAi.UAS.cUa, Scer\GAL4^esg.PU has increased cell number | somatic clone | adult stage phenotype

(Arthurton et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has adult midgut epithelium phenotype, suppressible | partially by phyl^GD12579, Scer\GAL4^NP5130

(Yin and Xi, 2018)

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has enteroendocrine cell | adult stage phenotype, suppressible by phyl^GD12579, Scer\GAL4^NP5130

(Yin and Xi, 2018)

NOT suppressed by

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^NP5130 has intestinal stem cell | adult stage phenotype, non-suppressible by phyl^GD12579, Scer\GAL4^NP5130

(Yin and Xi, 2018)

NOT Suppressor of

Statement

Reference

N^RNAi.UAS.cUa, Scer\GAL4^dpp.blk1 is a non-suppressor of wing disc phenotype of Pvr^{λ.UASp.Tag:MYC}, Scer\GAL4^dpp.blk1

(Wang et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Adult posterior midgut clones specifically generated in esg-positive progenitors (ISCs and EBs), and which both express N^{dsRNA.UAS.cUa} under the control of Scer\GAL4^esg.PU and are Dronc^{KO.-FRT.SunTag(Unk),Tag:HA,mCherry}/Dronc^KO transheterozygous show increased proportion of progenitors.

(Arthurton et al., 2020)

The abnormal adult midgut enteroendocrine cell-like tumors, but not the intestinal stem cell-like tumors, resulting from the expression of N^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^esg-NP5130 (using Gal80[ts] for the temporal control of expression) are suppressed by the co-expression of phyl^GD12579.

(Yin and Xi, 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

N^RNAi.UAS.cUa

N^{dsRNA.Scer\UAS.cUa}

N^{dsRNA.UAS.cUa}

notch^RNAi

(Cai et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections

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