FlyBase Allele Report: Scer\GAL4[Ir76b.916]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Scer\GAL4^Ir76b.916

General Information

Symbol

Scer\GAL4^Ir76b.916

Species

S. cerevisiae

Name

FlyBase ID

FBal0264269

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{Ir76b-GAL4.916}

Also Known As

IR76b-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Silbering et al., 2011)

Progenitor genotype

Carried in construct

P{Ir76b-GAL4.916}

Cytology

Description

A 916bp fragment from immediately upstream of the Ir76b start codon is fused upstream of sequence encoding a GAL4 driver.

(Silbering et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

antennal coeloconic sensillum ac1

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac2

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac3

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac4

(Silbering et al., 2011)

antennal lobe glomerulus VM1

(Silbering et al., 2011)

antennal lobe glomerulus VM4

(Silbering et al., 2011)

antennal lobe glomerulus VC3

(Silbering et al., 2011)

antennal lobe glomerulus VM6

(Silbering et al., 2011)

leg taste bristle | subset

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic leg taste bristle chemosensory neuron

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic tarsal segment 2

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic tarsal segment 3

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic tarsal segment 4

(Ganguly et al., 2017)

sugar-sensing neuron of taste bristle of metathoracic tarsal segment 5

(Ganguly et al., 2017)

pharyngeal sense organ

(Ganguly et al., 2017)

labellar taste peg

(Ganguly et al., 2017)

gustatory receptor neuron | subset of labellar taste bristle

(Ganguly et al., 2017)

gustatory receptor neuron of adult abdomen

(Sánchez-Alcañiz et al., 2018)

gustatory receptor neuron of wing

(Sánchez-Alcañiz et al., 2018)

mesothoracic leg taste bristle chemosensory neuron | subset

(Sánchez-Alcañiz et al., 2018)

metathoracic leg taste bristle chemosensory neuron | subset

(Sánchez-Alcañiz et al., 2018)

prothoracic leg taste bristle chemosensory neuron | subset

(Sánchez-Alcañiz et al., 2018)

dorsal cibarial sense organ neuron

(Sánchez-Alcañiz et al., 2018)

ventral cibarial sense organ neuron

(Sánchez-Alcañiz et al., 2018)

labral sense organ neuron

(Sánchez-Alcañiz et al., 2018)

gustatory receptor neuron of labellar taste bristle

(Sánchez-Alcañiz et al., 2018)

gustatory receptor neuron of labellar taste peg

(Sánchez-Alcañiz et al., 2018)

adult antennal olfactory receptor neuron | subset

(Sánchez-Alcañiz et al., 2018)

neuron of labellum | restricted

(Hussain et al., 2016)

neuron of adult antennal lobe | restricted

(Hussain et al., 2016)

neuron of leg | restricted

(Hussain et al., 2016)

wing margin | restricted

(Hussain et al., 2016)

Additional Information

Statement

Reference

ScerGAL4^Ir76b.916 drives expression in ~24 S cells in metathoracic tarsal segment 5 and ~11 in metathoracic tarsal segment 3. The remaining metathoracic leg tarsal segments show sex specific differences, with tarsal segment 4 having ~14 neurons in females and ~22 in males, while tarsal segment 2 has ~10 in females and ~15 in males. Expression near completely overlaps with that of ScerGAL4^Ir76b.1.5, except that ScerGAL4^Ir76b.916 does not label L1 GRNs in the labellar taste bristles, some cells in the labellum nor axonal projections in the adult subesophageal zone that are labelled by the former.

(Ganguly et al., 2017)

ScerGAL4^Ir76b.916 is expressed in the neurons that innervate the antennal coeloconic sensillum ac1-a4 and in the antennal lobe glomeruli VM1, VM4, VC3 and VC5.

(Silbering et al., 2011)

Marker for

antennal lobe glomerulus VC3

(Silbering et al., 2011)

antennal lobe glomerulus VM1

(Silbering et al., 2011)

antennal lobe glomerulus VM4

(Silbering et al., 2011)

antennal lobe glomerulus VM6

(Silbering et al., 2011)

Reflects expression of

(Sánchez-Alcañiz et al., 2018, Ganguly et al., 2017, Hussain et al., 2016, Silbering et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of Scer\GAL4^Ir76b.916 cells in adult females significantly increases walking speed, but not angular speed, compared to baseline, whereas no increase is seen in controls.

(Aso et al., 2014)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Ir76b.916/Agam\Ir76b^UAS.cGa is a suppressor of abnormal feeding behavior | adult stage phenotype of Ir76b²

(Ganguly et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Ir76b.916/Ir76b^UAS.cZa rescues Ir76b¹

(Croset et al., 2016)

Scer\GAL4^Ir76b.916/Ir76b^UAS.cZa rescues Ir76b¹

(Hussain et al., 2016)

Partially rescues

Scer\GAL4^Ir76b.916/Ir76b^UAS.cAa partially rescues Ir76b²

(Ganguly et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

41730

w^*; P{Ir76b-GAL4.916}226.8; TM2/TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^Ir76b.916

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections