FlyBase Allele Report: Dmel\cbt[NIG.4427R]

General Information

Symbol

Dmel\cbt^NIG.4427R

Species

D. melanogaster

Name

FlyBase ID

FBal0265997

Feature type

allele

Associated gene

Dmel\cbt

Associated Insertion(s)

Carried in Construct

P{NIG.4427R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Rodriguez, 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Rodriguez, 2011)

Mutagen

in vitro construct

(Rodriguez, 2011)

Progenitor genotype

Carried in construct

P{NIG.4427R}

Cytology

Description

UASt regulatory sequences drive expression of a cbt RNAi construct.

(Rodriguez, 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-4427R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | conditional, with Scer\GAL4^αTub84B.PL

(Havula et al., 2013)

partially lethal | conditional, with Scer\GAL4^αTub84B.PL

(Havula et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of cbt^NIG.4427R under the control of Scer\GAL4^αTub84B.PL (using a temperature sensitive Scer\GAL80 allele to limit expression to the larval stage) results in a modest delay in pupation on a low sugar diet. Hemolymph glucose levels are not significantly affected in the mutant larvae. Animals raised on a 20% yeast, 15% sucrose diet show a prominent developmental delay and impaired survival.

(Havula et al., 2013)

Expression of cbt^NIG.4427R under the control of Scer\GAL4^nos.PG or Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} does not generate an observable phenotype.

(Belacortu et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

cbt^NIG.4427R, Scer\GAL4^esg.PU is a suppressor | partially of increased occurrence of cell division | adult stage phenotype of E2f1^UASp.EGFP, Scer\GAL4^esg.PU