Embryos with maternal expression of zip^GL00623 under the control of Scer\GAL4^{VP16.mat.αTub67C} displayed highly penetrant axis elongation defects and die. The stage 7 ventrolateral germband exhibits decreased retraction velocity along the AP edges but not DV edges in laser ablation experiments), as compared to rescued controls; during cell intercalation, the contraction rate of anterior-posterior edges and the number of completely contracted edges is reduced compared to rescued controls; there are ectopic tissue folds; cortical F-actin does not seem to show any obvious defects.

These embryos do not show changes in germ band cell area, cell aspect ratio, or rate of directional vertex resolution to form DV edges during cell intercalation, as compared with rescued controls. However, adding zip^{R707C.sqh.GFP} leads to a decrease in the rate of vertex resolution, and increased cell elongation, still without affecting the apical cell area; and adding zip^N98K.sqh.GFP leads to increased cell elongation parallel to the DV axis, still without affecting the apical cell area.

Adult flies expressing zip^GL00623 under the control of Scer\GAL4^Dot.PK display nephrocyte functional defects (measured by uptake assays) and reduced adult lifespan relative to controls.

zip^GL00623, Scer\GAL4^GMR.PF is a suppressor of visible | adult stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

zip^GL00623, Scer\GAL4^GMR.PF is a suppressor of melanotic necrosis | adult stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF