FB2025_01 , released February 20, 2025
Allele: Dmel\Nlg4LL01874
Open Close
General Information
Symbol
Dmel\Nlg4LL01874
Species
D. melanogaster
Name
FlyBase ID
FBal0290286
Feature type
allele
Associated gene
Dmel\Nlg4
Associated Insertion(s)
PBac{SAstopDsRed}Nlg4LL01874
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Li et al., 2013)
Progenitor genotype
Associated Insertion(s)
PBac{SAstopDsRed}Nlg4LL01874
Cytology
Description
Allele components
Component
Use(s)
Inserted element
PBac{SAstopDsRed}
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Nlg4LL01874 mutant flies show significantly less total night sleep time and longer sleep onset latency, compared with control flies. Compared with wild-type, these mutants exhibit more night sleep episodes, with markedly shorter durations, reflecting poorly consolidated night sleep in Nlg4LL01874 mutants. Similar results are seen in Nlg4LL01874/Nlg4del transheterozygotes and Nlg4LL01874/Df(3R)BSC516 and Nlg4LL01874/Df(3R)BSC808 flies.

      Large ventral clock neurons (l-LNvs) in Nlg4LL01874 mutant flies show similar electrophysiological properties as those from wild-type flies during the day, with tonic spontaneous AP firing and frequent spike bursts. l-LNvs in Nlg4LL01874 mutants are hyper-activated at night.

      In Nlg4LL01874 mutant flies, l-LNv dendrite branches are distributed in a disordered fashion. Nlg4LL01874 mutant flies have a fewer varicosities in the optic lobe compared with wild-type flies.

      (Li et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Nlg4LL01874 has abnormal sleep phenotype, suppressible by RdlMD-RR

      (Li et al., 2013)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The introduction of a RdlR-VL background successfully restores night sleep in Nlg4LL01874 mutants, indicating that the sleep defects observed in Nlg4LL01874 mutants are due to impaired GABA transmission.

      (Li et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Nlg4LL01874 is rescued by Nlg4UAS.cLa/Scer\GAL4P2.4.Pdf

      (Li et al., 2013)

      Nlg4LL01874 is rescued by Scer\GAL4dimm-929/Nlg4UAS.cLa

      (Li et al., 2013)
      Not rescued by

      Nlg4LL01874 is not rescued by Scer\GAL4104Y/Nlg4UAS.cLa

      (Li et al., 2013)

      Nlg4LL01874 is not rescued by Nlg4UAS.cLa/Scer\GAL4GMR.PF

      (Li et al., 2013)
      Comments

      Sleep defects, especially those in early evening sleep, in Nlg4LL01874 mutants, are restored through expression of Nlg4Scer\UAS.cLa in l-LNvs using Scer\GAL4P2.4.Pdf or Scer\GAL4dimm-929. However, expression in the fan-shaped body neurons (under the control of Scer\GAL4104Y) or under the control of Scer\GAL4GMR.PF fails to rescue sleep defects in Nlg4LL01874 mutants.

      (Li et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nlg4LL01874
      dnlg4
      (Li et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)