FB2025_01 , released February 20, 2025
Allele: Dmel\Gr28bUAS.D
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General Information
Symbol
Dmel\Gr28bUAS.D
Species
D. melanogaster
Name
FlyBase ID
FBal0291409
Feature type
allele
Associated gene
Dmel\Gr28b
Associated Insertion(s)
Carried in Construct
P{UAS-Gr28b.D}
Also Known As
UAS-Gr28b.d
Key Links
Transgenic product class
wild_type
(Ni et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Ni et al., 2013)
Mutagen
in vitro construct
(Ni et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-Gr28b.D}
Cytology
Description

UAS regulatory sequences drive expression of the Gr28b D isoform.

(Ni et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Gr28b
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Gr28bScer\UAS.D under the control of Scer\GAL4Act5C.PU become incapacitated when placed at 37[o]C for three minutes, recovering when returned to 23[o]C.

      Expression of Gr28bScer\UAS.D under the control of Scer\GAL4Gr5a.8.5 produces gustatory bristle receptor responses in response to warming. Warming to 32[o]C also produces a proboscis extension response.

      Unlike in wild type, expression of Gr28bScer\UAS.D in motor neurons under the control of Scer\GAL4VGlut-OK371 induces warmth-responsive excitatory junction potentials at the neuromuscular junction. Motor neurons expressing Gr28bScer\UAS.D under the control of Scer\GAL4Mhc.PW exhibit warmth-responsive inward currents.

      (Ni et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Gr28bScer\UAS.D in the arista under the control of Scer\GAL4Gr28b.d.PT significantly rescues the "rapid negative thermotaxis" defects seen in TrpA1ins mutant flies.

      (Ni et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Gr28bUAS.D/Scer\GAL4Gr28b.d.PT rescues Gr28bMB03888

      (Ni et al., 2013)

      Gr28bUAS.D/Scer\GAL4hot-cell rescues Gr28bMB03888

      (Ni et al., 2013)
      Comments

      Expression of Gr28bScer\UAS.D under the control of either Scer\GAL4Gr28b.d.PT or Scer\GAL4hot-cell rescues the "rapid negative thermotaxis" defects seen in Gr28bMB03888 mutant flies.

      (Ni et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Gr28bScer\UAS.D
      Gr28bUAS.D
      Name Synonyms
      Secondary FlyBase IDs
        References (5)