FB2025_01 , released February 20, 2025
Allele: Dmel\Mcm10RNAi.UAS.633-700
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General Information
Symbol
Dmel\Mcm10RNAi.UAS.633-700
Species
D. melanogaster
Name
FlyBase ID
FBal0295684
Feature type
allele
Associated gene
Dmel\Mcm10
Associated Insertion(s)
Carried in Construct
P{UAS-Mcm10.RNAi.633-700}
Key Links
Transgenic product class
RNAi_reagent
(Vo et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Vo et al., 2014)
Mutagen
in vitro construct
(Vo et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-Mcm10.RNAi.633-700}
Cytology
Description

UAS regulates expression of a cDNA fragment encoding amino acids 633-700 of Mcm10 in normal and reversed directions to generate expression of dsRNA.

(Vo et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Mcm10
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4GMR.PU-mediated expression of Mcm10dsRNA.Scer\UAS.633-700 results in a rough eye, reduced ommatidial number, and an overall smaller eye.

      Third instar eye discs expressing Mcm10dsRNA.Scer\UAS.633-700 via Scer\GAL4GMR.PU show a wider than normal zone of S phase cells, indicating more cells are delayed in S phase, and a more diffuse area and increased number of mitotic cells, indicating delayed M phase entry and progression.

      Scer\GAL4GMR.PU-mediated expression of Mcm10dsRNA.Scer\UAS.633-700 results in more phospho-H2AvD positive cells in the posterior region of eye discs, indicating DNA damage.

      Scer\GAL4GMR.PU-mediated expression of Mcm10dsRNA.Scer\UAS.633-700 results in a significant increase in cell death signals in the region posterior to the morphogenetic furrow.

      Scer\GAL4GMR.PU-mediated expression of Mcm10dsRNA.Scer\UAS.633-700 specifically inhibits the differentiation of R7 photoreceptor cells.

      (Vo et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of BacA\p35Scer\UAS.cHa suppresses the ectopic induction of apoptotic signals and partially suppresses the rough eye phenotype of Scer\GAL4GMR.PU Mcm10dsRNA.Scer\UAS.633-700 animals. However, the inhibition of R7 differentiation resulting from Mcm10dsRNA.Scer\UAS.633-700 expression is not suppressed.

      (Vo et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Mcm10RNAi.UAS.633-700
      Mcm10dsRNA.Scer\UAS.633-700
      Mcm10dsRNA.UAS.633-700
      Name Synonyms
      Secondary FlyBase IDs
        References (2)