FlyBase Allele Report: Dmel\MetRS[L262G.UAS.EGFP]

General Information

Symbol

Dmel\MetRS^{L262G.UAS.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0314188

Feature type

allele

Associated gene

Dmel\MetRS

Associated Insertion(s)

Carried in Construct

P{UAS-MetRS.L262G.EGFP}

Also Known As

UAS-dMetRS^L262G-EGFP

Key Links

Transgenic product class

missense_variant

(Erdmann et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Erdmann et al., 2015)

Mutagen

in vitro construct

(Erdmann et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-MetRS.L262G.EGFP}

Cytology

Description

UASt regulates expression of MetRS containing a leucine to glycine substitution within the MetRS-binding pocket (which enables incorporation of the non-canonical amino acid azidonorleucine) and tagged with EGFP.

(Erdmann et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

MetRS

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^VGlut-OK371-mediated expression does not affect innervation of muscle 24.

(Niehues et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^ppk.PU is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Hsap\GARS1^E71G.UAS, Scer\GAL4^ppk.PU

(Niehues et al., 2015)

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^VGlut1-OK371 is a non-enhancer of abnormal neuroanatomy phenotype of Hsap\GARS1^G240R.UAS, Scer\GAL4^VGlut1-OK371

(Niehues et al., 2015)

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^ppk.PU is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Hsap\GARS1^G240R.UAS, Scer\GAL4^ppk.PU

(Niehues et al., 2015)

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^VGlut1-OK371 is a non-enhancer of abnormal neuroanatomy phenotype of Hsap\GARS1^G526R.UAS, Scer\GAL4^VGlut1-OK371

(Niehues et al., 2015)

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^ppk.PU is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Hsap\GARS1^G526R.UAS, Scer\GAL4^ppk.PU

(Niehues et al., 2015)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^ppk.PU is a non-enhancer of larval multidendritic class IV neuron | larval stage phenotype of Hsap\GARS1^E71G.UAS, Scer\GAL4^ppk.PU

(Niehues et al., 2015)

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^VGlut1-OK371 is a non-enhancer of embryonic/larval neuromuscular junction phenotype of Hsap\GARS1^G240R.UAS, Scer\GAL4^VGlut1-OK371

(Niehues et al., 2015)

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^ppk.PU is a non-enhancer of larval multidendritic class IV neuron | larval stage phenotype of Hsap\GARS1^G240R.UAS, Scer\GAL4^ppk.PU

(Niehues et al., 2015)

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^VGlut1-OK371 is a non-enhancer of embryonic/larval neuromuscular junction phenotype of Hsap\GARS1^G526R.UAS, Scer\GAL4^VGlut1-OK371

(Niehues et al., 2015)

MetRS^{L262G.UAS.EGFP}, Scer\GAL4^ppk.PU is a non-enhancer of larval multidendritic class IV neuron | larval stage phenotype of Hsap\GARS1^G526R.UAS, Scer\GAL4^ppk.PU

(Niehues et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

MetRS^{L262G.Scer\UAS.T:Avic\GFP-EGFP}

MetRS^{L262G.UAS.EGFP}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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