FB2025_01 , released February 20, 2025
Allele: Dmel\NO66KO
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General Information
Symbol
Dmel\NO66KO
Species
D. melanogaster
Name
FlyBase ID
FBal0335409
Feature type
allele
Associated gene
Dmel\NO66
Associated Insertion(s)
TI{TI}NO66KO
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Shalaby et al., 2017)
Mutagen
ends-out gene targeting
(Shalaby et al., 2017)
FLPase
(Shalaby et al., 2017)
I-SceI
(Shalaby et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}NO66KO
Cytology
Description

The endogenous NO66 open reading frame has been replaced by a loxP cassette which contains both Disc\RFP3xP3.PC and the kanamycin resistance gene.

(Shalaby et al., 2017)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:4,319,628..4,321,589 [+]
Comment:

The NO66 ORF was replaced with a knock-in cassette containing a Red Fluorescent Protein (RFP) transgene. Replaced region determined from reported primer sequences.

(Shalaby et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  alcohol use disorder
      CEA
      (Pinzón et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      NO66KO mutants are viable and fertile and do not display any developmental timing defects. NO66KO suppresses the variegated phenotype of the w or Sb locus in the In(1)wm4 or T(2;3)SbV background, respectively.

      (Shalaby et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Statement
      Reference

      NO66[+]/NO66KO is a non-enhancer of visible | adult stage phenotype of Scer\GAL4C-765, smo5A.UAS.Tag:FLAG

      (Shalaby et al., 2017)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      NO66[+]/NO66KO is a non-suppressor of visible | adult stage phenotype of Scer\GAL4C-765, smo5A.UAS.Tag:FLAG

      (Shalaby et al., 2017)
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      NO66[+]/NO66KO is a non-enhancer of wing vein phenotype of Scer\GAL4C-765, smo5A.UAS.Tag:FLAG

      (Shalaby et al., 2017)
      Suppressor of
      Statement
      Reference

      NO66[+]/NO66KO is a suppressor | partially of eye phenotype of Scer\GAL4ey.200.Exel, TAF1BRNAi.UAS

      (Shalaby et al., 2017)

      NO66[+]/NO66KO is a suppressor of eye phenotype of Scer\GAL4GMR.PF, ykiS168A.UAS.EGFP,Tag:HA

      (Shalaby et al., 2017)
      NOT Suppressor of
      Statement
      Reference

      NO66[+]/NO66KO is a non-suppressor of wing vein phenotype of Scer\GAL4C-765, smo5A.UAS.Tag:FLAG

      (Shalaby et al., 2017)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Combination with a single copy of NO66KO decreases the frequency of eye tissue to antenna-like malformed structures transformation in adult flies expressing TAF1BdsRNA.Scer\UAS under the control of Scer\GAL4ey.200.Exel, partially suppresses the massive eye overgrowth in flies expressing ykiS168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 with Scer\GAL4GMR.PF but does not significantly affect the disrupted wing vein phenotype of flies expressing smo5A.Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4C-765.

      (Shalaby et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      NO66KO
      (Shalaby et al., 2018, Pinzón et al., 2017, Shalaby et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)