A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Allele Dmel\smo5A.Scer\UAS.T:Zzzz\FLAG

General Information
SymbolDmel\smo5A.Scer\UAS.T:Zzzz\FLAGSpeciesD. melanogaster
NameFlyBase IDFBal0177836
Feature typealleleAssociated geneDmel\smo
Allele class
Mutagenin vitro construct - regulatory fusionin vitro construct - coding region fusionin vitro construct - amino acid replacementin vitro construct - site directed mutagenesis
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Description
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FB2013_03
FB2013_02
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Allele class
Mutagen
Mutations Mapped to the Genome
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion
Statement
Reference
Construct: Amino acid replacement: S667A. Amino acid replacement: S687A. Amino acid replacement: S740A. Amino acid replacement: T755A. Amino acid replacement: T785A. Nucleotide substitution: T2237G. Nucleotide substitution: T2287G. Nucleotide substitution: A2368G. Nucleotide substitution: G2369C. Nucleotide substitution: A2501A. Nucleotide substitution: A2539G. Scer\UAS regulatory sequences drive expression of a mutated form of smo in which the five putative PKA phosphorylation sites in the C-terminal, cytoplasmic tail have been mutated. The smo protein is tagged with T:Zzzz\FLAG at the C-terminal end.
Carried in construct
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Cytology
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Statement
Reference
Expression of smo5A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4C-765 results in a reduction in the distance between veins III and IV in the wing and a partial fusions of these veins proximal to the anterior crossvein. The reduction in distance between wing veins III and IV and partial fusions of these veins proximal to the anterior crossvein that is seen in flies expressing smo5A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4C-765 are strongly enhanced by smo3/+.
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Statement
Reference
The reduction in distance between wing veins III and IV and partial fusions of these veins proximal to the anterior crossvein that is seen in flies expressing smo5A.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4C-765 are suppressed by ptcG12/+; the resulting flies have nearly normal wings.
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Bloomington
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Reported As
Symbol Synonym
smo5A.Scer\UAS.T:Zzzz\FLAG
 
Name Synonym
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Mao and Freeman, 2009, Development 136(3): 473--481
Fasciclin 2, the Drosophila orthologue of neural cell-adhesion molecule, inhibits EGF receptor signalling. [FBrf0206540]
Casso et al., 2008, Genetics 178(3): 1399--1413
A screen for modifiers of hedgehog signaling in Drosophila melanogaster identifies swm and mts. [FBrf0205418]
Bejarano et al., 2007, EMBO Rep. 8(8): 778--783
Hedgehog restricts its expression domain in the Drosophila wing. [FBrf0200898]
Collins and Cohen, 2005, Genetics 170(1): 173--184
A genetic screen in Drosophila for identifying novel components of the hedgehog signaling pathway. [FBrf0188527]
Personal communication to FlyBase
Cook, 2007.7.24, P{UAS-smo.5A}2.
P{UAS-smo.5A}2. [FBrf0200086]
Collins, 2005.7.7, Genetics 2005 170:173-185.
Genetics 2005 170:173-185. [FBrf0188528]