ci, Su(fu) Physical Interaction report

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

bait

GST tag

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Monnier et al., 1998)

FBrf0102486-5.coIP.WB

Reference

Monnier et al., 1998

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

bait

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Monnier et al., 1998)

FBrf0105294-2.Y2H

Reference

Paumard-Rigal et al., 1998

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

fused to LexA DNA-binding domain

Su(fu)

prey

fused to B42 activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Paumard-Rigal et al., 1998)

Two-hybrid system: yeast LexA-BD/B42-AD

(Paumard-Rigal et al., 1998)

Positive control.

(Paumard-Rigal et al., 1998)

FBrf0128807-11.coIP.WB

Reference

Stegman et al., 2000

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

bait

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of cl8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Stegman et al., 2000)

FBrf0128807-7.PD.WB

Reference

Stegman et al., 2000

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

Su(Fu)

bait

GST tag

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from cell extract of baculovirus infected Sf21 cell line.

(Stegman et al., 2000)

FBrf0132087-1.PD.A

Reference

Methot and Basler, 2000

Description

physical association

Assay

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

bait

GST tag

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

N-terminal region

sufficient binding region

aa 5-440

region

necessary binding region

aa 212-268

region

mutation decreasing interaction

aa 268-346

deletion decreases the interaction

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Methot and Basler, 2000)

FBrf0167876-17.coIP.WB

Reference

Lum et al., 2003

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

bait

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of clone8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lum et al., 2003)

Su(fu) interacts more strongly with ci than with cos or fu.

(Lum et al., 2003)

FBrf0174526-3a.coIP.WB

Reference

Wang and Jiang, 2004

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

HA tag

Su(fu)

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Ci76

sufficient binding region

aa 1-703

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Wang and Jiang, 2004)

FBrf0174526-7.PD.A

Reference

Wang and Jiang, 2004

Description

physical association

Assay

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

GST tag

Su(fu)

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Ci76

sufficient binding region

aa 1-703

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Wang and Jiang, 2004)

cos and Su(fu) compete for binding to the N-terminal region of ci.

(Wang and Jiang, 2004)

FBrf0174526-9.coIP.WB

Reference

Wang and Jiang, 2004

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

HA tag

Su(fu)

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

cos and Su(fu) compete for binding to the N-terminal region of ci.

(Wang and Jiang, 2004)

Su(fu) protein detected with antibody to endogenous protein.

(Wang and Jiang, 2004)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang and Jiang, 2004)

FBrf0194555-7.coIP.WB

Reference

Zhang et al., 2006

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

Myc tag

Su(fu)

Sufu

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2006)

Su(fu) and rdx compete for binding to ci.

(Zhang et al., 2006)

FBrf0208973-1.coIP.WB

Reference

Farzan et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

SuFu

bait

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of Cl8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Farzan et al., 2009)

FBrf0208973-4.CH.WB

Reference

Farzan et al., 2009

Description

physical association

Assay

molecular sieving

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

SuFu

neutral component

Su(fu)

SuFu

neutral component

Su(fu)

SuFu

neutral component

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of Cl8 cell line; proteins produced from endogenous genes.

(Farzan et al., 2009)

FBrf0216910-6.coIP.WB

Reference

Shi et al., 2011

Description

physical association

Assay

Collection

Source/Stage

Cell line used

fluorescent resonance energy transfer

Participants

Corresponds to

Reported as

Role

Note

bait

Myc tag, PKA sites mutated

Su(fu)

Sufu

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

The ci construct used contains Serine-to-Alanine mutations in three PKA phosphorylation sites to prevent proteolytic processing.

(Shi et al., 2011)

This ci-Su(fu) interaction is inhibited by the co-expression of activated fu constructs.

(Shi et al., 2011)

Bait expressed in transiently transfected cells.

(Shi et al., 2011)

Prey expressed in transiently transfected cells.

(Shi et al., 2011)

FBrf0216910-7.FRET

Reference

Shi et al., 2011

Description

physical association

Assay

Collection

Source/Stage

Cell line used

isothermal titration calorimetry

Participants

Corresponds to

Reported as

Role

Note

bait

CFP tag, PKA sites mutated

Su(fu)

Sufu

prey

YFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

The ci construct used contains Serine-to-Alanine mutations in three PKA phosphorylation sites to prevent proteolytic processing.

(Shi et al., 2011)

Interaction inferred from the ability of excited CFP to induce YFP fluorescence.

(Shi et al., 2011)

This ci-Su(fu) interaction is inhibited by the co-expression of activated fu constructs.

(Shi et al., 2011)

Bait expressed in transiently transfected cells.

(Shi et al., 2011)

Prey expressed in transiently transfected cells.

(Shi et al., 2011)

FBrf0223216-1.ITC

Reference

Zhang et al., 2013

Description

physical association

Assay

predetermined participant

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

Su(fu)

prey

His tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

sufficient binding region

aa 230-272

interaction domain

mutation disrupting interaction

aa 257-259

G257A,H258A,I259D

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait peptide synthesized in vitro; prey produced as a recombinant fusion protein in baculovirus and insect cell system.

(Zhang et al., 2013)

FBrf0224245-3.PD.WB

Reference

Shi et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

CiN

bait

GST tag

Su(fu)

Sufu

prey

HA tag

Su(fu)

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

N-terminal region

sufficient binding region

aa 1-440

coordinates relative to ci-PA

Su(fu)

SYGH motif binding pocket residue D154

necessary binding region

aa 154

D154R, coordinates relative to Su(fu)-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Trn and Su(fu) compete for binding to ci. Functional experiments suggest that Su(fu) binding of ci inhibits Trn-mediated nuclear import.

(Shi et al., 2014)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey immunopurified from transfected cell extract.

(Shi et al., 2014)

FBrf0227573-2.Y2H

Reference

Paces-Fessy et al., 2004

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

dSufu

bait

fused to LexA DNA-binding domain

dCi

prey

fused to B42A activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Paces-Fessy et al., 2004)

Two-hybrid system: yeast LexA-BD/B42A-AD

(Paces-Fessy et al., 2004)

FBrf0229286-3.coIP.WB

Reference

Oh et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

FLAG tag

Su(fu)

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

necessary binding region

aa 1-346

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction requires the expression of cos RNAi.

(Oh et al., 2015)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Oh et al., 2015)

FBrf0229286-4.coIP.WB

Reference

Oh et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

bait

V5 tag

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

necessary binding region

aa 1-346

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction requires the expression of cos RNAi.

(Oh et al., 2015)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Oh et al., 2015)

FBrf0229673-10.coIP.WB

Reference

Zhou et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

Myc tag

Su(fu)

Sufu

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

The Su(fu)-ci interaction is suppressed by cotransfection of Usp7.

(Zhou et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhou et al., 2015)

FBrf0232221-1.coIP.WB

Reference

Han et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

Myc tag

Su(fu)

prey

FLAG tag

Su(fu)

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

sufficient binding region

aa 1-439aa 440-1160aa 621-1020aa 1161-1397

coordinates relative to ci-PA

interaction domain

mutation decreasing interaction

aa 1371-1397aa 213-259

coordinates relative to ci-PA

interaction domain

mutation disrupting interaction

aa 213-259,1371-1397

coordinates relative to ci-PA

Su(fu)

interaction domain residue 154

mutation disrupting interaction

aa 154

This mutation disrupts interaction with ci fragment 1-439 but not with ci fragment 1161-1397.

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Han et al., 2015)

Mutant ci with three PKA sites mutated to Ala used for some of the interaction studies. Studies indicate multiple regions of ci interact with multiple regions of Su(fu).

(Han et al., 2015)

FBrf0232221-2.PD.WB

Reference

Han et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

GST tag

Su(fu)

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

sufficient binding region

aa 1-439aa 1371-1397

strong binding, coordinates relative to ci-PA

weak binding, coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from cell extract of transfected S2 cells.

(Han et al., 2015)

FBrf0232221-3.coIP.WB

Reference

Han et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

bait

FLAG tag

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

sufficient binding region

aa 1-439aa 1161-1397

coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Han et al., 2015)

Studies indicate multiple regions of ci interact with Su(fu). Lack of competition indicates multiple regions of ci interact with multiple regions of Su(fu).

(Han et al., 2015)

FBrf0232221-5.coIP.WB

Reference

Han et al., 2015

Description

physical association

Assay

Collection

Source/Stage

wing disc / larval stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

Myc tag

Su(fu)

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

mutation decreasing interaction

aa 1371-1397aa 213-259

coordinates relative to ci-PA

interaction domain

mutation disrupting interaction

aa 213-259,1371-1397

coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Han et al., 2015)

FBrf0245753-3.coIP.WB

Reference

Han et al., 2019

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-ThermoFischer

Participants

Corresponds to

Reported as

Role

Note

bait

Myc tag

Su(fu)

Sufu

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

phosphorylation target residues 218,220

mutation decreasing interaction

aa 218,220

S218D,S220D, coordinates relative to ci-PA

phosphorylation target residues 1230,1233

mutation decreasing interaction

aa 1230,1233

S1230D,S1233D, coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Han et al., 2019)

ci-Su(fu) interaction assessed with a form of ci with PKA site mutations S838A, S856A, S892A.

(Han et al., 2019)

FBrf0245753-4.coIP.WB

Reference

Han et al., 2019

Description

physical association

Assay

fluorescent resonance energy transfer

Collection

Source/Stage

Cell line used

S2-ThermoFischer

Participants

Corresponds to

Reported as

Role

Note

Su(fu)

Sufu

bait

HA tag

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

ci-Su(fu) interaction assessed with a form of ci with PKA site mutations S838A, S856A, S892A.

(Han et al., 2019)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Han et al., 2019)

FBrf0245753-6.FRET.FM

Reference

Han et al., 2019

Description

physical association

Assay

fluorescence microscopy

Collection

Source/Stage

Cell line used

S2-ThermoFischer

Participants

Corresponds to

Reported as

Role

Note

fluorescence donor

CFP tag

fluorescence donor

CFP tag

fluorescence donor

CFP tag

Su(fu)

Sufu

fluorescence acceptor

YFP tag

Su(fu)

Sufu

fluorescence acceptor

YFP tag

Su(fu)

Sufu

fluorescence acceptor

YFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

phosphorylation target residues 218,220,1230,1233

mutation decreasing interaction

aa 218,220,1230,1233

S218D,S220D,S1230D,S1233D coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

ci-Su(fu) interaction assessed with a form of ci with PKA site mutations S838A, S856A, S892A. Coexpression of fu interferes with the ci-Su(fu) interaction in a manner dependent on its kinase activity.

(Han et al., 2019)

Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct.

(Han et al., 2019)

FBrf0254867-4.PD.WB

Reference

Zhou et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

GST tag

Su(fu)

Sufu

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

C-terminal domain

mutation decreasing interaction

aa 1382, 1384 and 1385

S1282D, T1384D and S1385D phospho-mimetic mutations; within the context of the C-terminal fragment aa 1371-1385.

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct in SR2+ cell line.

(Zhou et al., 2022)

FBrf0254867-5.coIP.WB

Reference

Zhou et al., 2022

Description

physical association

Assay

Collection

Source/Stage

Cell line used