Reference Report

Reference
Citation	Haines, N., Irvine, K.I. (2005). Functional analysis of Drosophila 1,4-N-acetlygalactosaminyltransferases. Glycobiology 15(4): 335--346. (Export to RIS)
FlyBase ID	FBrf0182892
Publication Type	Research paper
PubMed ID	15563714
PubMed Abstract	Members of the mammalian beta1,4-galactosyltransferase family are among the best studied glycosyltransferases, but the requirements for all members of this family within an animal have not previously been determined. Here, we describe analysis of two Drosophila genes, beta4GalNAcTA (CG8536) and beta4GalNAcTB (CG14517), that are homologous to mammalian beta1,4-galactosyltransferases. Like their mammalian homologs, these glycosyltransferases use N-acetylglucosamine as an acceptor substrate. However, they transfer N-acetylgalactosamine rather than galactose. This activity, together with amino acid sequence similarity, places them among a group of recently identified invertebrate beta1,4-N-acetylgalactosaminyltransferases. To investigate the biological functions of these genes, null mutations were generated by imprecise excision of a transposable element (beta4GalNAcTA) or by gene-targeted homologous recombination (beta4GalNAcTB). Flies mutant for beta4GalNAcTA are viable and fertile but display behavioral phenotypes suggestive of essential roles for GalNAc-beta1,4-GlcNAc containing glycoconjugates in neuronal and/or muscular function. beta4GalNAcTB mutants are viable and display no evident morphological or behavioral phenotypes. Flies doubly mutant for both genes display only the behavioral phenotypes associated with mutation of beta4GalNAcTA. Thus Drosophila homologs of the mammalian beta4GalT family are essential for neuromuscular physiology or development but are not otherwise required for viability, fertility, or external morphology.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Glycobiology
Title	Glycobiology
Publication Year	1990-
ISBN/ISSN	0959-6658
Data from Reference
Aberrations (1)
	Df(2R)β4GalNAcTA^20.1
Alleles (13)
	w^+* w^+mC β4GalNAcTA^4.1 β4GalNAcTA^7.1 β4GalNAcTA^+t2.4 β4GalNAcTA^EP2551 β4GalNAcTA^{MtnA.T:SV5\V5,T:Zzzz\His6} β4GalNAcTB^{BIP.MtnA.T:SV5\V5,T:Zzzz\His6} β4GalNAcTB^BIP.MtnA β4GalNAcTB^EndsOut.H β4GalNAcTB^GT β4GalNAcTB^MtnA.PH β4GalNAcTB^{MtnA.T:SV5\V5,T:Zzzz\His6}
Constructs (2)
	P{EndsOut-β4GalNAcTB.H} P{β4GalNAcTA^+t2.4}
Genes (6)
	CG8531 T:SV5\V5 T:Zzzz\His6 w β4GalNAcTA β4GalNAcTB
Insertions (1)
	P{EP}β4GalNAcTA^EP2551