FlyBase Insertion Report: Dmel\P{PZ}Tm1[02299]

General Information

Symbol

Dmel\P{PZ}Tm1⁰²²⁹⁹

Species

D. melanogaster

Name

FlyBase ID

FBti0005454

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)02299

Affected gene(s)

Ecol\lacZ, Tm1

Viability / fertility

Associated allele(s)

Ecol\lacZ^Tm1-02299, Tm1⁰²²⁹⁹

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 88E12 )

Sequence Location

3R:15,291,441..15,291,441 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:15,291,441..15,291,441 [+]

(Tetzlaff et al., 1996)

Orientation

Cytological location (computed by FlyBase)

88E12 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Harvie et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Tm1

(Tetzlaff et al., 1996)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Tm1-02299

Phenotypic Class Phenotype Manifest in Detailed Description

Tm1⁰²²⁹⁹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage -- third instar larval stage

corpus allatum

(Harvie et al., 1998)

prothoracic gland

(Harvie et al., 1998)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval foregut

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

embryonic/larval salivary gland

(Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

larval ventral nerve cord

(Harvie et al., 1998)

Malpighian tubule

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

During second-third instar larval stages, expressed in lateral secretory cells (prothoracic gland) and medial secretory cells (corpus allatum) of the ring gland. Expression in larval CNS exhibits a punctate pattern.

(Harvie et al., 1998)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)02299

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11564

ry⁵⁰⁶ P{PZ}Tm1⁰²²⁹⁹/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

The orientation of the P element was not reported.

(Tetzlaff et al., 1996)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1564

(Meister and Braun, 1995.10)

P{PZ}Tm1⁰²²⁹⁹

(Tetzlaff et al., 1996, FlyBase, 1992-)

P{PZ}l(3)02299⁰²²⁹⁹

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)02299⁰²²⁹⁹

(FlyBase, 1992-)

TmII^c

(Li and Gao, 2003)

Name Synonyms

Secondary FlyBase IDs

FBti0000514

References (16)

Report Sections

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