FB2025_01 , released February 20, 2025
Insertion: Dmel\P{PZ}Acsl07447
Open Close
General Information
Symbol
Dmel\P{PZ}Acsl07447
Species
D. melanogaster
Name
FlyBase ID
FBti0005990
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
LINE ID

l(2)07447

Affected gene(s)
Acsl, Ecol\lacZ
Viability / fertility
Associated allele(s)
Acsl07447, Ecol\lacZl(2)44DEa-07447
Stock availability
None publicly available
Genomic Location
Chromosomal Location
2R ( 44E1-44E2 )
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
44E1-44E2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AF006647
(FlyBase, 1992-)
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene
Acsl
(BDGP Project Members, 1994-1999)
Ecol\lacZ
(Harvie et al., 1998, BDGP Project Members, 1994-1999)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
second instar larval stage -- third instar larval stage
ring gland
(Harvie et al., 1998)
wandering third instar larval stage
embryonic/larval optic lobe
(Harvie et al., 1998)
larval ventral nerve cord
(Harvie et al., 1998)
Additional Information
Statement
Reference

During second instar to third instar larval stages, expressed in the ring gland, including in lateral secretory cells (prothoracic gland), the medial secretory cells (corpus allatum), and the corpus cardiacum cells. Expression in larval CNS exhibits a punctate pattern.

(Harvie et al., 1998)
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)07447

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{PZ}Acsl07447
P{PZ}l(2)0584707447
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}l(2)44DEa07447
(FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (8)