FlyBase Insertion Report: Dmel\P{GawB}amn[NP2397]

General Information

Symbol

Dmel\P{GawB}amn^NP2397

Species

D. melanogaster

Name

FlyBase ID

FBti0034828

Feature type

transposable_element_insertion_site

Also Known As

NP2397

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

amn, Scer\GAL4

Viability / fertility

Associated allele(s)

amn^NP2397, Scer\GAL4^NP2397

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

X ( 18F4-19A2 )

Sequence Location

X:19,886,743..19,887,128

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

X:19,886,743..19,887,128

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

18F4-19A2 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG218642

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

amn

(FlyBase Curators, 2008-)

Scer\GAL4

(Pauls et al., 2010, Tanaka et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

amn^NP2397

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^NP2397

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult dopaminergic PAM neuron

(Flybrain Neuron Database et al., 2018.6.28, Tanaka et al., 2008)

mushroom body gamma lobe slice 4

(Tanaka et al., 2008)

mushroom body beta' anterior-posterior layer

(Tanaka et al., 2008)

mushroom body beta' lobe slice 1

(Tanaka et al., 2008)

mushroom body beta' lobe slice 2

(Tanaka et al., 2008)

mushroom body beta' middle layer

(Tanaka et al., 2008)

Additional Information

Statement

Reference

ScerGAL4^amn-NP2397 drives expression in mushroom body extrinsic neurons, including the mushroom body anterior inferior medial cell and dopaminergic PAM neurons, including dopaminergic PAM neurons 13 and 14, that innervate mushroom body gamma lobe slice 3, mushroom body gamma lobe slice 4, mushroom body gamma lobe slice 5, mushroom body beta' lobe slice 1 and mushroom body beta' lobe slice 2. Expression partially overlaps with that driven by EcollexA^GMR24E12 in 12 dopaminergic PAM neurons 13 and 14.

(Shuai et al., 2015)

Projections of the mushroom body anterior inferior medial neurons innervate the anterior stratum of the beta' lobe segment 1, and/or throughout the middle and posterior strata of segment 2.

(Tanaka et al., 2008)

Marker for

adult antennal lobe projection neuron VL1 ilPN

(Tanaka et al., 2008)

Reflects expression of

amn

(Tanaka et al., 2008)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)