Recombinant construct P{UAS-Hsc70-3.WT}

General Information
Symbol	P{UAS-Hsc70-3.WT}	FlyBase ID	FBtp0011232
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Elefant and Palter, 1999)
Uses	CV term Qualifiers & info Reference binary system (targeted) Hsc70-3 (Elefant and Palter, 1999) characterization Hsc70-3 (Elefant and Palter, 1999)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Elefant and Palter, 1999) selectable marker (Elefant and Palter, 1999)
Component Alleles
Allele	Hsc70-3^Scer\UAS.cEa
Reference(s)	(Elefant and Palter, 1999, Yamakawa et al., 2012, Gruenewald et al., 2009)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of a 2.1kb EcoRI fragment containing the Hsc70-3 coding region. (Elefant and Palter, 1999)
Phenotypic class	viable, with Scer\GAL4^how-24B (Elefant and Palter, 1999)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Elefant and Palter, 1999)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 2 )
Bloomington	5843 w¹²⁶; P{UAS-Hsc70-3.WT}B
Kyoto	108461 w¹²⁶; P{UAS-Hsc70-3.WT}B
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	3/WT/UAS (Elefant and Palter, 1999) P{UAS-Hsc70-3.WT} (FlyBase, 1996-) P{UAS-Hsc70-3} (Gruenewald et al., 2009) UAS-Hsc70-3{wt} (Yamakawa et al., 2012)
Secondary FlyBase IDs

References ( 3 )
Research paper	Yamakawa et al., 2012, Development 139(3): 558--567 Deficient Notch signaling associated with neurogenic pecanex is compensated for by the unfolded protein response in Drosophila. [FBrf0217160] Gruenewald et al., 2009, Free Radical Biol. Med. 46(12): 1668--1676 Hyperoxia-induced neurodegeneration as a tool to identify neuroprotective genes in Drosophila melanogaster. [FBrf0208024] Elefant and Palter, 1999, Mol. Biol. Cell 10(7): 2101--2117 Tissue-specific expression of dominant negative mutant Drosophila HSC70 causes developmental defects and lethality. [FBrf0109657]