[25B1-25B1];[25B4-25B4];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
25B1;25B4
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Ready-to-hatch Df(2L)BSC182 homozygous larvae have an abnormal transparent appearance, their head skeleton is pale and their tracheal system is not filled with gas, as compared to controls.
Mutant larvae exhibit a defective cuticle, as shown by cuticle retraction (and consequent shorter larvae) in cuticle preparations, excessive permeability to Eosin Y, and severely decreased autofluorescence of first instar larvae upon exposure to 405nm light, despite no apparent changes in integument argentaffin (which labels the cuticulin layer) or Sudan black stainings, and a severely decreased integument Sudan black staining, as compared to controls.. At the ultrastructure level, however, all cuticle layers shows an apparently normal morphology, as compared to controls.
Flies heterozygous for the deletion do not show a Minute bristle phenotype.
The presence of P+PBac{XP5.WH5}BSC182 was verified using the PCR methods and primers described in FBrf0175003.
The cytological breakpoints of Df(2L)BSC182 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 25B1;25B4.