[47F8-47F8];[48B6-48B6];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
47F8;48B6
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
The presence of P+PBac{XP5.RB3}BSC231 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The
cytological breakpoints of Df(2R)BSC231 predicted from the Release 5 genomic coordinates of the progenitor transposable elements PBac{RB}sprte01563 and P{XP}d01948 insertion sites are 47F8;48B6.