A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of the deficiency in Df(1)BSC822 was confirmed by the failure to amplify a 520 base pair fragment spanning the PBac{WH}l(1)G0230f04497 (FBti0042358) insertion site from Df(1)BSC822/PBac{WH}l(1)G0230f04497 flies using primers 5'-CTGAAGCTATCTAAACGAGACTTAACCACG-3' and 5'-GCATTATCATATCAACGGTCTATCCCAC-3'.
The breakpoints of Df(1)BSC822 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}CG32698e03908 and P{XP}CG2909d05081 transposable element insertion sites are X:9732123 ;10222698 and the cytological breakpoints predicted from these coordinates are 9A2;9B7.