A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of the deficiency was verified by showing that a 895 base pair fragment spanning the P{EPgy2}EY22844 insertion site could not be amplified from Df(3R)BSC861/P{EPgy2}EY22844 flies using primers 5'-GCCAGTTGGTTTGTCATGTTTGCC-3' and 5'-GAGCGTTCTGGTCACTTTCGGG-3'.