FlyBase Allele Report: Dmel\cn[3]

General Information

Symbol

Dmel\cn³

Species

D. melanogaster

Name

FlyBase ID

FBal0001734

Feature type

allele

Associated gene

Dmel\cn

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

Mutagen

Progenitor genotype

Cytology

Description

0.5kb deletion and a 7.5kn insertion located about 5kb distally.

(Warren et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Campesan et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color

(Maddison et al., 2020)

abnormal eye color | recessive

abnormal locomotor behavior | adult stage

(Maddison et al., 2020)

short lived

(Maddison et al., 2020)

Phenotype Manifest In

eye

(Maddison et al., 2020)

mitochondrion

(Maddison et al., 2020)

ocellus

pigment cell

Detailed Description

Statement

Reference

Synthesis of 3-hydroxykynurenine (3-HK) is essentially eliminated in cn³ mutant flies at day one post-eclosion, whereas kynurenic acid (KYNA) levels increased, leading to a dramatic decrease in the 3-HK/KYNA ratio.

(Campesan et al., 2011)

RK1.

(Craymer, 1980)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

cn³ has abnormal eye color phenotype, suppressible by Hsap\KMO^UAS.cMa/Scer\GAL4^Act5C.PI

(Maddison et al., 2020)

cn³ has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Drp1^{Tag:FLAG,Tag:CALI(TC),Tag:HA}

(Maddison et al., 2020)

Suppressor of

Statement

Reference

cn³ is a suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Campesan et al., 2011)

Other

Statement

Reference

cn³, park²⁵ has partially lethal - majority die phenotype

(Maddison et al., 2020)

Pink1^B9, cn³ has partially lethal - majority die phenotype

(Maddison et al., 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

cn³/cn³ is an enhancer of adult thorax phenotype of Pink1^B9

(Maddison et al., 2020)

Suppressor of

Statement

Reference

cn³/cn³ is a suppressor | partially of adult thorax phenotype of park²⁵

(Maddison et al., 2020)

cn³ is a suppressor of rhabdomere phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Campesan et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Homozygous cn³ partially suppresses the rhabdomere degeneration seen in one day old flies expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} under the control of Scer\GAL4^elav-C155. Some rescue, albeit reduced, is also seen at seven days post-eclosion. No rescue is seen with heterozygous cn³. The increase in 3-hydroxykynurenine (3-HK)/kynurenic acid (KYNA) ratio is also significantly suppressed, with flies exhibiting ratios that are similar to those seen in cn³ mutant flies alone. Unlike when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed alone, treating these flies with Ro 61-8048 is unable to further rescue the rhabdomere degeneration. Feeding the flies 3-HK suppresses the protective effect of cn³ in a dose-dependent manner; at the highest dose the level of rhabdomere degeneration is similar to that seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed alone. The 3-HK/KYNA ratio is also restored.

(Campesan et al., 2011)

Complementation and Rescue Data

Rescued by

cn³ is rescued by Scer\GAL4^Act5C.PI/cn^UAS.cMa

(Maddison et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

105824

cn³/Tp(2;Y)C

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

cn³

(Maddison et al., 2020, Breda et al., 2016, Campesan et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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