The salivary gland placodes of fas2 embryos appear identical to wild type at the gross morphological level, but the cells in the placode are stacked upon each other in two or three rows and are variably elongated (in contrast to wild type where the placode consists of a monolayer of uniformly elongated cells). At the stage when the dorsal-posterior pit forms in the salivary gland placode in wild-type embryos, a slight indentation is seen near the centre of the placode in fas2 embryos, suggestive of cell shape changes. The invaginating pit is not as deep or wide as in wild-type embryos. Cells at the centre of the pit appear elongated with basally positioned nuclei, however, the surrounding cells in the pit are round and found in multiple layers. Cells in the anterior and posterior parts of the gland also form multi-layered placodes. Although the initial indentation occurs at slightly variable locations in different embryos of similar age, the pit observed in late-stage mutant embryos is trough-shaped and uniformly located close to the centre of the placode. The salivary glands are eventually internalised, despite gross abnormalities in cell shape. The internalised gland is comprised of a mixture of elongated and wedge-shaped cells. Cells in the anterior and posterior parts of the gland are multi-layered. After internalisation, the salivary glands fuse into one dome-shaped organ which is close to the ventral surface. The gland is multi-layered and has the remnants of a potentially contiguous lumen. When internalisation is complete, the surface morphology of the salivary pit is similar to wild type.
Cardioblasts do not align properly with their ipsilateral partners in homozygous embryos and often fail to migrate far enough to reach their contralateral counterparts. The number of pericardiocytes is reduced compared to wild-type, and those that are present tend to migrate away from the dorsal midline.
Malpighian tubules arrest early in their morphogenesis in homozygous embryos. The tubules are typically spherical, although slight lengthening of the tubules occurs in some embryos. The tip cells are present.
The movement and shape of sensilla is abnormal in homozygous embryos. At stage 16, sensory neurons are located at the surface of the embryo, in contrast to wild-type, where they are located subepidermally at this stage. Epidermal cells surrounding the sensilla do not form regular monolayered sheets, but pile up into 2-3 layers of irregularly shaped cells. Accessory cells of the sensilla fail to form lateral processes that wrap around the sensory dendrite, and they do not form the apical processes that become the shaft and socket of the sensillum. Condensation of the ventral nerve cord does not occur in homozygous embryos. The commissures do not differentiate. Midline cells do not form the regular, dense clusters typical of wild-type embryos.
The 'fas[1]' EMS allele and the 'P{PZ}05488' insertion were previously associated with the CG17716 gene, named 'faint sausage (fas)' (Lekven et al, 1998; FBrf0103312). However, a subsequent study has shown these alleles affect a different gene 'CG6197/fand' (Sauerwald et al, 2017; FBrf0234822). As far as can be ascertained, the 'fas[2]' (aka 'fas[IIA]') allele was defined by its failure to complement fas[1] and P{PZ}05488 (FBrf0041708, FBrf0103312) and so this allele has also been made an allele of CG6197/fand.