FlyBase Allele Report: Dmel\fs(1)Yb[1]

General Information

Symbol

Dmel\fs(1)Yb¹

Species

D. melanogaster

Name

FlyBase ID

FBal0004496

Feature type

allele

Associated gene

Dmel\fs(1)Yb

Associated Insertion(s)

Carried in Construct

Also Known As

Yb¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lin and Wolfner, 1989)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G2916A. Amino acid replacement: R?Q.

(King and Lin, 1999)

No defect detectable by Southern blotting in the 8.5kb SalI fragment corresponding to that included in P{fs-1}.

(Lin and Wolfner, 1989)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,723,381..2,723,381 [+]

Nucleotide change:

G2723381A

Reported nucleotide change:

G2916A

Amino acid change:

R898Q | fs(1)Yb-PA

Reported amino acid change:

R?Q

(King and Lin, 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | oogenesis

(King et al., 2001)

decreased fecundity | female | recessive

lethal - all die during embryonic stage | maternal effect | recessive

some die during embryonic stage | recessive | maternal effect | recessive

Phenotype Manifest In

egg | maternal effect

egg chamber

(King and Lin, 1999)

follicle cell

(King et al., 2001)

germarium

(King and Lin, 1999)

germline stem cell

(King et al., 2001)

ovariole

(King and Lin, 1999)

Detailed Description

Statement

Reference

In mutant ovarioles, the number of follicle cells and germ cells is reduced. This is not due to massive cell death.

(King et al., 2001)

Homozygous third larval instar ovaries have a normal overall morphology, and have the normal number and location of germline stem cells. Ovarioles of newly eclosed homozygous females generally have 1-3 egg chambers (instead of 5 as in wild type). 42% have no germline cells, 39% have undifferentiated germline cells and 17% have differentiated germline cells in the germarium. Ovarioles of newly eclosed fs(1)Yb¹/Df(1)w258-45 females generally have 1-3 egg chambers (instead of 5 as in wild type). 8% have no germline cells, 5% have undifferentiated germline cells and 88% have differentiated germline cells in the germarium. Germline clonal analysis indicates that fs(1)Yb is not required in the germline for fertility.

(King and Lin, 1999)

Semi-sterile in females, and causes a less severe effect on ovary morphology than fs(1)Ya⁴ or fs(1)Ya⁶.

(Johnson et al., 1995)

Females lay reduced numbers of eggs, most of which remain vacuolated after deposition; a few develop into normal-appearing adults. No effect on males.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

fs(1)Yb¹ has female sterile phenotype, non-suppressible by hh^hs.PI

(King et al., 2001)

Other

Statement

Reference

fs(1)Yb¹, hh^hs.PI has increased cell number | oogenesis phenotype

(King et al., 2001)

fs(1)Yb¹, hh^hs.PI has increased occurrence of cell division | oogenesis phenotype

(King et al., 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

fs(1)Yb¹ has germline stem cell phenotype, non-enhanceable by piwi¹

(King et al., 2001)

Suppressed by

Statement

Reference

fs(1)Yb¹ has germarium phenotype, suppressible by hh^hs.PI

(King et al., 2001)

NOT suppressed by

Statement

Reference

fs(1)Yb¹ has germline stem cell phenotype, non-suppressible by piwi¹

(King et al., 2001)

NOT Enhancer of

Statement

Reference

fs(1)Yb¹ is a non-enhancer of germline stem cell phenotype of piwi¹

(King et al., 2001)

NOT Suppressor of

Statement

Reference

fs(1)Yb¹ is a non-suppressor of germline stem cell phenotype of piwi¹

(King et al., 2001)

Other

Statement

Reference

fs(1)Yb¹, hh^hs.PI has stalk follicle cell | increased number phenotype

(King et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Heat-shocking fs(1)Yb¹ heterozygotes that carry one or two copies of the hh^hs.PI transgene induces the formation of large stalks composed of overproliferated somatic cells. Heat-shocking fs(1)Yb¹ homozygotes (with hh^hs.PI) induces the formation of large interfollicular stalks. Heat-shocking fs(1)Yb¹ homozygotes with one or two copies of hh^hs.PI, increases the number of egg chambers per ovariole, and increases the number of morphologically normal germaria in a dose dependant manner. Fertility is not restored.

(King et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

fs(1)Yb¹ is partially rescued by fs(1)Yb^t6.4

(King and Lin, 1999)

Not rescued by

fs(1)Yb¹ is not rescued by fs(1)Yb^t1.8

(King and Lin, 1999)

fs(1)Yb¹ is not rescued by fs(1)Yb^t3.9

(King and Lin, 1999)

fs(1)Yb¹ is not rescued by fs(1)Yb^t7.7

(King and Lin, 1999)

fs(1)Yb¹ is not rescued by fs(1)Yb^t7.2

(King and Lin, 1999)

fs(1)Yb¹ is not rescued by fs(1)Yb^t4.6

(King and Lin, 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Yb¹

(Olivieri et al., 2010, Smulders-Srinivasan et al., 2010, Szakmary et al., 2009, Szakmary and Lin, 2002)

Yb^1co

(King and Lin, 1999)

fs(1)Y¹

fs(1)Yb¹

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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