FB2026_02 , released June 18, 2026
Allele: Dmel\wupAfliH
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General Information
Symbol
Dmel\wupAfliH
Species
D. melanogaster
Name
FlyBase ID
FBal0005434
Feature type
allele
Associated gene
Dmel\wupA
Associated Insertion(s)
Carried in Construct
Also Known As
hdp102
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Lindsley and Zimm, 1992)
Progenitor genotype
Cytology
Description

Sequencing of the wupA-coding region as well as untranslated regions (UTRs) of messenger RNA does not reveal any mutation. Sequencing reveals no mutation in the intronic regulatory element (IRE) and few changes in the upstream regulatory element (URE). Changes include a TTA insertion at one site, and changes in another site that include insertion of a T at one location and deletion of a T from a nearby sequence.

(Firdaus et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:18,117,731..18,117,731 [-]
Comment:

One base deleted at this location. Third of three changes in the wupA upstream region detected in this mutant.

(Firdaus et al., 2015)
insertion
X:18,117,723..18,117,724 [-]
Comment:

One base (A on the - strand) are inserted at this location. Second of three changes in the wupA upstream region detected in this mutant.

(Firdaus et al., 2015)
insertion
X:18,117,684..18,117,685 [-]
Comment:

Three bases (TAA on the - strand) are inserted at this location. First of three changes in the wupA upstream region detected in this mutant.

(Firdaus et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      wupAfliH mutant flies are completely flightless. The dorsal longitudinal muscles (DLMs) show phenotypes related to abnormal hypercontraction. In the mutants, muscles are torn from the middle with few remnants attached at both ends. The muscle hypercontraction phenotype of wupAfliH is temperature-dependent. DLM morphology is completely ameliorated in mutant flies raised at lower temperature.

      The jumping and walking ability of wupAfliH mutant flies is also found to be significantly compromised compared to wild-type, suggesting that the tergal depressor of trochanter (TDT) and leg muscles are also affected. This phenotype is not ameliorated at 18[o]. Rather the phenotype is more pronounced at this temperature than at 25[o]. The jumping and walking abilities of mutant flies do not show any deterioration when monitored for several days post-eclosion, ruling out any enhanced age-dependent progressive

      wupAfliH leads to partial lethality at various stages of development at 25[o]C compared to wild-type. At 18[o]C, lethality is much reduced.

      The DLM fiber morphology is completely rescued by T(1;Y)B18 and Dp(1;Y)W39. These flies also show slight rescue of the flight ability.

      wupAfliH fails to complement wupAhdp-3 and all the flies show muscle tearing and hypercontraction.

      (Firdaus et al., 2015)

      Homozygotes are flightless, and have a normal wing posture. Double heterozygotes with upint-3 or up101 have greatly reduced flight ability.

      (Homyk and Emerson, 1988)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      up101, fliH[+]/wupAfliH has flightless phenotype

      (Firdaus et al., 2015)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      wupAfliH has dorsal longitudinal indirect flight muscle cell | heat sensitive phenotype, suppressible by Mhc[+]/Mhc2B

      (Firdaus et al., 2015)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Trans-heterozygous wupAfliH, up101 flies show a gradation of phenotypes from flightless to individuals with normal flight. None of these flies show any muscle defects.

      (Firdaus et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      wupAhdp-2

      (Firdaus et al., 2015)

      wupAhdp-3

      (Firdaus et al., 2015)
      Rescued by

      wupAfliH is rescued by wupAUAS.L9

      (Firdaus et al., 2015)
      Comments

      wupAfliH fails to complement wupAhdp-3 and all the flies show muscle tearing and hypercontraction.

      Targeted overexpression of wupAScer\UAS.L9 under the control of Scer\GAL4Hk-UH3, which expresses in developing indirect flight muscles (IFMs), brings about rescue of the wupAfliH muscle phenotype. Six dorsal longitudinal muscles (DLMs) are seen with normal banding pattern sarcomeres. Rescued flies also show functional recovery of the muscles as indicated by their flight ability.

      (Firdaus et al., 2015)

      Shows partial complementation over Bxhdp-a-2.

      (Lindsley and Zimm, 1992, Homyk and Emerson, 1988)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      References (6)