73% of Pgant35A¹/Pgant35A⁴ homozygous embryos hatch to larvae, 73% of the larvae pupate, but no pupae eclose.

87% of Pgant35A¹/Pgant35A³ homozygous embryos hatch to larvae, 69% of the larvae pupate, but no pupae eclose.

Pgant35A¹/Pgant35A³ animals derived from a cross of females carrying homozygous Pgant35A¹ germ-line clones to Pgant35A³/+ males show considerable lethality; only 12% of embryos hatch as larvae and none of the larvae pupate.

Pgant35A¹/+ animals derived from a cross of females carrying homozygous Pgant35A¹ germ-line clones to Pgant35A³/+ males show some lethality; 45% of embryos hatch as larvae, 17% of the larvae pupate, and 93% of the pupae eclose.

Most Pgant35A¹/Pgant35A³ embryos that are lacking both maternal and zygotic Pgant35A⁺ function show normal tracheal development at stage 13. However, by stage 17 they show obvious tracheal defects; in particular, the dorsal longitudinal trunk is convoluted and follows a circuitous path and is highly irregular in diameter. The tracheal cells are either much smaller or larger than wild type and are highly irregular in shape. The hindgut and salivary glands have normal morphology in these embryos. When the embryos are injected with 10kDa dextran dye, the dye is found within the dorsal tracheal tubes in the majority of cases (in contrast to wild-type embryos), indicating that diffusion barrier formation is disrupted in the trachea in these embryos.