FB2026_02 , released June 18, 2026
Allele: Dmel\Pgant35A3
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General Information
Symbol
Dmel\Pgant35A3
Species
D. melanogaster
Name
FlyBase ID
FBal0008456
Feature type
allele
Associated gene
Dmel\Pgant35A
Associated Insertion(s)
Carried in Construct
Also Known As
l(2)35AaSF12
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Ashburner et al., 1999.10.12, Woodruff and Ashburner, 1979)
Progenitor genotype
Cytology
Description

Nucleotide substitution: ?584A.

(Schwientek et al., 2002)

Nucleotide substitution: T584A.

(Ten Hagen and Tran, 2002)

Amino acid replacement: ?195term.

(Schwientek et al., 2002, Ten Hagen and Tran, 2002)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:14,359,963..14,359,963 [+]
Nucleotide change:

T14359963A

Reported nucleotide change:

T584A

Amino acid change:

L195term | Pgant35A-PA

Reported amino acid change:

?195term

(Schwientek et al., 2002, Ten Hagen and Tran, 2002)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      87% of Pgant35A1/Pgant35A3 homozygous embryos hatch to larvae, 69% of the larvae pupate, but no pupae eclose.

      80% of Pgant35A4/Pgant35A3 homozygous embryos hatch to larvae, 64% of the larvae pupate, but no pupae eclose.

      Pgant35A1/Pgant35A3 animals derived from a cross of females carrying homozygous Pgant35A1 germ-line clones to Pgant35A3/+ males show considerable lethality; only 12% of embryos hatch as larvae and none of the larvae pupate.

      Pgant35A4/Pgant35A3 animals derived from a cross of females carrying homozygous Pgant35A4 germ-line clones to Pgant35A3/+ males show considerable lethality; only 12% of embryos hatch as larvae and none of the larvae pupate.

      Most Pgant35A4/Pgant35A3 and Pgant35A1/Pgant35A3 embryos that are lacking both maternal and zygotic Pgant35A+ function show normal tracheal development at stage 13. However, by stage 17 they show obvious tracheal defects; in particular, the dorsal longitudinal trunk is convoluted and follows a circuitous path and is highly irregular in diameter. The tracheal cells are either much smaller or larger than wild type and are highly irregular in shape. The hindgut and salivary glands have normal morphology in these embryos. When the embryos are injected with 10kDa dextran dye, the dye is found within the dorsal tracheal tubes in the majority of cases (in contrast to wild-type embryos), indicating that diffusion barrier formation is disrupted in the trachea in these embryos.

      (Tian and Ten Hagen, 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Pgant35A3/Df(2L)b84h1 is rescued by Pgant35A+t3.8

      (Bennett et al., 2010)

      Pgant35A3/Df(2L)b84h1 is rescued by Pgant35AUAS.cBa/Scer\GAL4arm.PS

      (Bennett et al., 2010)

      Pgant35A3/Df(2L)b84h1 is rescued by Pgant35Ahs.PB

      (Bennett et al., 2010)

      Pgant35A3/Pgant35A4 is rescued by Pgant35A+tGal

      (Flores and Engels, 1999)
      Partially rescued by

      Pgant35A3/Pgant35A4 is partially rescued by Scer\GAL4btl.PS/Pgant35AUAS.cTa

      (Tian and Ten Hagen, 2007)
      Not rescued by

      Pgant35A3/Df(2L)b84h1 is not rescued by Pgant35Atmut

      (Bennett et al., 2010)
      Comments

      Pgant35A+t3.8 rescues the lethality of Pgant35A3/Df(2L)b84h1.

      Pgant35Atmut fails to rescue the lethality of Pgant35A3/Df(2L)b84h1.

      Expression of Pgant35AScer\UAS.cBa under the control of Scer\GAL4arm.PS rescues the lethality of Pgant35A3/Df(2L)b84h1.

      Expression of Pgant35Ahs.PB at 25[o]C rescues the lethality of Pgant35A3/Df(2L)b84h1.

      (Bennett et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      Ashburner.

      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      3775
      (Ten Hagen and Tran, 2002)
      Pgant35A3
      SF12
      l(2)35Aa3
      (Ten Hagen and Tran, 2002, Ashburner et al., 1999.10.12)
      l(2)35AaSF12
      (Schwientek et al., 2002, Flores and Engels, 1999)
      l(2)br12SF12
      (Woodruff and Ashburner, 1979)
      pgant35A3775
      (Tian and Ten Hagen, 2007)
      pgant35A3
      (Bennett et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)