FlyBase Allele Report: Dmel\Dna2[D2]

General Information

Symbol

Dmel\Dna2^D2

Species

D. melanogaster

Name

FlyBase ID

FBal0012615

Feature type

allele

Associated gene

Dmel\Dna2

Associated Insertion(s)

Carried in Construct

Also Known As

mus109^D2

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Mason et al., 1981)

Progenitor genotype

Cytology

Description

A nonsense mutation truncating the protein at the end of the 'stalk' domain.

(Mitchell et al., 2022)

Amino acid replacement: Q583term.

(Mitchell et al., 2022)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:10,012,433..10,012,433 [+]

Nucleotide change:

C10012433T

Amino acid change:

Q583term | Dna2-PB; Q582term | Dna2-PC

Reported amino acid change:

Q583term

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Mitchell et al., 2022)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair

(Mitchell et al., 2022)

abnormal DNA repair (with Df(1)BSC539)

(Mitchell et al., 2022)

abnormal DNA repair (with Df(1)BSC754)

(Mitchell et al., 2022)

abnormal DNA repair (with Df(1)ED6989)

(Mitchell et al., 2022)

abnormal DNA repair (with Df(1)ED6991)

(Mitchell et al., 2022)

abnormal DNA repair (with Dna2^D1)

(Mitchell et al., 2022)

abnormal DNA repair (with Dna2^lS)

(Mitchell et al., 2022)

abnormal mitotic cell cycle | recessive

(Gatti, 1979)

chemical sensitive

(Mason et al., 1981)

female sterile | recessive

(Mason et al., 1981)

increased mortality | chemical conditional

(Mitchell et al., 2022)

increased mortality | chemical conditional (with Df(1)BSC539)

(Mitchell et al., 2022)

increased mortality | chemical conditional (with Df(1)BSC754)

(Mitchell et al., 2022)

increased mortality | chemical conditional (with Df(1)ED6989)

(Mitchell et al., 2022)

increased mortality | chemical conditional (with Df(1)ED6991)

(Mitchell et al., 2022)

increased mortality | chemical conditional (with Dna2^D1)

(Mitchell et al., 2022)

increased mortality | chemical conditional (with Dna2^lS)

(Mitchell et al., 2022)

Phenotype Manifest In

mitosis & nuclear chromosome

(Gatti, 1979)

Detailed Description

Statement

Reference

Dna2^D2/Dna2^D2, Dna2^D1/Dna2^D2, Dna2^D2/Dna2^lS, Dna2^D2/Df(1)BSC539, Dna2^D2/Df(1)BSC754, Dna2^D2/Df(1)ED6989 and Dna2^D2/Df(1)ED6991 individuals show increased sensitivity to the mutagen MMS.

(Mitchell et al., 2022)

mus109^D2 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32^oC or 38^oC.

(Wei and Rong, 2007)

Sterile.

(Boyd and Shaw, 1982)

Hemizygous male larvae are not sensitive to HN2. They are sensitive to 0.08% MMS but relatively insensitive to 0.04% MMS. This phenotype is slightly stronger than that of mus109^A1.

(Mason et al., 1981)

Homozygotes and hemizygotes show a higher frequency of spontaneous chromosome aberrations in neuroblast metaphases than wild-type larvae. The ratio of chromatid breaks to isochromatid breaks is 0.7-1.0. Approximately 80% of the breaks are heterochromatic. The breaks appear to be randomly distributed among the chromosomes. The frequency of breaks is the same in females and males.

(Gatti, 1979)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Dna2^unspecified

(Gatti, 1979)

Comments

Fails to complement mus109^unspecified for female sterility.

(Gatti, 1979)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2307

sn³ Dna2^D2/C(1)DX, y¹ f¹

Notes on Origin

Discoverer

Mason.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dna2^D2

mus-107^D2

mus-109^D2

(Gatti, 1979)

mus109^D2

(Mitchell et al., 2022, Wei and Rong, 2007)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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