FlyBase Allele Report: Dmel\mus304[D2]

General Information

Symbol

Dmel\mus304^D2

Species

D. melanogaster

Name

FlyBase ID

FBal0012648

Feature type

allele

Associated gene

Dmel\mus304

Associated Insertion(s)

Carried in Construct

Also Known As

mus304²

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Boyd et al., 1981)

Progenitor genotype

Cytology

Description

Amino acid replacement: W759term.

(Brodsky et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:18,112,342..18,112,342 [+]

Nucleotide change:

G18112342A

Amino acid change:

W759term | mus304-PA

Reported amino acid change:

W759term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified)

(Brodsky et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive | recessive

(Boyd et al., 1981)

visible | recessive

(Brodsky et al., 2000)

Phenotype Manifest In

macrochaeta

(Brodsky et al., 2000)

Detailed Description

Statement

Reference

mus304^D2 mutant cells exhibit DNA breaks and telomere fusions in metaphase and anaphase at almost the same level as wild-type cells.

(Oikemus et al., 2006)

mus304^D2 animals show a strong defect in a DNA damage checkpoint assay in the eye disc. mus304^D2/Df(3L)W4 animals have macrochaetae defects (probably due genomic instability resulting in the loss of genetic material at haploinsufficient Minute loci). mus304^D1/mus304^D2 flies have wild-type eyes. Loss of heterozygosity is elevated compared to wild-type in mus304^D2/Df(3L)W4 animals.

(Brodsky et al., 2000)

Hypersensitive to methyl methanesulfonate. Sensitive to 0.008% HN2.

(Boyd et al., 1981)

female-sterile

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mus304^D2 has abnormal mitotic cell cycle phenotype, enhanceable by tefu¹

(Oikemus et al., 2006)

mus304^D2 has abnormal mitotic cell cycle phenotype, enhanceable by nbs¹

(Oikemus et al., 2006)

Df(3L)W4/mus304^D2 has visible phenotype, enhanceable by E(mus304)[+]/PolA1^Emus304

(Brodsky et al., 2000)

Enhancer of

Statement

Reference

mus304^D2 is an enhancer of abnormal mitotic cell cycle phenotype of tefu¹

(Oikemus et al., 2006)

mus304^D2 is an enhancer of abnormal mitotic cell cycle phenotype of nbs¹

(Oikemus et al., 2006)

Other

Statement

Reference

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has visible phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has visible phenotype

(Brodsky et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(3L)W4/mus304^D2 has macrochaeta phenotype, enhanceable by E(mus304)[+]/PolA1^Emus304

(Brodsky et al., 2000)

Other

Statement

Reference

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has macrochaeta phenotype

(Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has eye phenotype

(Brodsky et al., 2000)

E(mus304)[+]/PolA1^Emus304, mus304^D1/mus304^D2 has ommatidium phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has macrochaeta phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has eye phenotype

(Brodsky et al., 2000)

PolA1^Emus304, mus304^D1/mus304^D2 has ommatidium phenotype

(Brodsky et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

nbs¹ mus304^D2 double mutant cells exhibit more DNA breaks and telomere fusions in metaphase and anaphase than in wild-type cells.

tefu¹ mus304^D2 double mutant cells exhibit more DNA breaks and telomere fusions in metaphase and anaphase than in wild-type cells.

(Oikemus et al., 2006)

The frequency of macrochaetae defects in mus304^D2/Df(3L)W4 animals is increased by one copy of E(mus304)¹. If mus304^D1/mus304^D2 flies are also heterozygous for E(mus304)¹ they have rough eyes and abnormal or missing bristles.

(Brodsky et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Boyd.

Separable from: E(mus304)¹.

(Brodsky et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mus304²

(Abdu et al., 2002, Brodsky et al., 2000)

mus304^D2

(Oikemus et al., 2006, Brodsky et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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