FlyBase Allele Report: Dmel\Polr2A[12]

General Information

Symbol

Dmel\Polr2A¹²

Species

D. melanogaster

Name

FlyBase ID

FBal0014669

Feature type

allele

Associated gene

Dmel\Polr2A

Associated Insertion(s)

Carried in Construct

Also Known As

E28

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Chen et al., 1993, Voelker et al., 1985)

Progenitor genotype

Polr2A⁴

(Chen et al., 1993, Voelker et al., 1985, Mortin and Kaufman, 1982)

Cytology

Description

Nucleotide substitution: G2880A.

(Krasnoselskaya et al., 1998)

Amino acid replacement: A377T. Nucleotide substitution: G2880A.

(Chen et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,568,239..11,568,239 [-]

Nucleotide change:

G11568239A

Reported nucleotide change:

G2880A

Amino acid change:

A377T | Polr2A-PA

Reported amino acid change:

A377T

(Chen et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant

(Voelker et al., 1985)

lethal | heat sensitive

(Mortin, 1990)

lethal | recessive

(Kim et al., 1994)

lethal | recessive | heat sensitive

(Krasnoselskaya et al., 1998, Chen et al., 1993, Lindsley and Zimm, 1992, Voelker et al., 1985)

Phenotype Manifest In

capitellum

(Chen et al., 1993)

Detailed Description

Statement

Reference

Selected for loss of α-amanitin resistance.

(Krasnoselskaya et al., 1998)

Exhibits the Ubx effect: heterozygotes partially transform the capitellum of the haltere into the wing blade.

(Chen et al., 1993)

Haplospecific lethal. Survives, at least with a low frequency, as a homozygote, but is lethal in hemizygotes.

(Lindsley and Zimm, 1992)

Mutant flies show resistance to α-amanitin.

(Voelker et al., 1985)

Temperature-sensitive mutation.

(Mortin and Kaufman, 1982)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Polr2A¹² has lethal phenotype, suppressible by Polr2B^S7

(Kim et al., 1994)

Polr2A¹² has lethal phenotype, suppressible by Polr2B^S11

(Kim et al., 1994)

Polr2A¹² has lethal phenotype, suppressible by Polr2B^S12

(Kim et al., 1994)

Polr2A¹² has lethal phenotype, suppressible by Polr2B^M

(Kim et al., 1994)

NOT suppressed by

Statement

Reference

Polr2A¹² has lethal phenotype, non-suppressible by Polr2B^S1

(Kim et al., 1994)

Polr2A¹² has lethal phenotype, non-suppressible by Polr2B^S2

(Kim et al., 1994)

Polr2A¹² has lethal phenotype, non-suppressible by Polr2B^S5

(Kim et al., 1994)

Polr2A¹² has lethal phenotype, non-suppressible by Polr2B^S6

(Kim et al., 1994)

Polr2A¹² has lethal phenotype, non-suppressible by Polr2B^S8

(Kim et al., 1994)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Polr2A¹² has phenotype, non-suppressible by Polr2B^SM

(Krasnoselskaya et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The recessive lethality of RpII215¹² is not rescued by one copy of RpII140^S1, RpII140^S2, RpII140^S5, RpII140^S6 or RpII140^S8. The recessive lethality of RpII215¹² is rescued to between 1 and 49% viability by one copy of RpII140^S7, RpII140^S11 or RpII140^S12. The recessive lethality of RpII215^K1 is suppressed by RpII140^M.

(Kim et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

3661

v¹ Polr2A¹²/FM7a/Dp(1;Y)y⁺v⁺#3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

E28

(Chen et al., 1993, Voelker et al., 1985)

Polr2A¹²

RpII215¹²

(Popodi et al., 2010-)

RpII215^E28

(Lindsley and Zimm, 1992, Mortin, 1990)

RpII^E28

(Mortin and Kaufman, 1982)

Ubl^E28

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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