FlyBase Allele Report: Dmel\Polr2A[K1]

General Information

Symbol

Dmel\Polr2A^K1

Species

D. melanogaster

Name

FlyBase ID

FBal0014775

Feature type

allele

Associated gene

Dmel\Polr2A

Associated Insertion(s)

Carried in Construct

Also Known As

WJK1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Chen et al., 1993, Mortin et al., 1988)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G3784A.

(Krasnoselskaya et al., 1998)

Amino acid replacement: S678N. Nucleotide substitution: G3784A.

(Chen et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,567,335..11,567,335 [-]

Nucleotide change:

G11567335A

Reported nucleotide change:

G3784A

Amino acid change:

S678N | Polr2A-PA

Reported amino acid change:

S678N

(Chen et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Mortin, 1990)

lethal | recessive

(Kim et al., 1994)

lethal | recessive | heat sensitive

(Krasnoselskaya et al., 1998, Mortin et al., 1988)

viable

(Chen et al., 1993)

Phenotype Manifest In

Detailed Description

Statement

Reference

Fails to exhibit the Ubx effect: heterozygotes fail to partially transform the capitellum of the haltere into the wing blade.

(Chen et al., 1993)

Homozygous flies die at 29^oC and survive at 19^oC.

(Mortin et al., 1988)

temperature-sensitive recessive lethal flies survive at 19^oC but not at 29^oC. Although not displaying the Ubx-enhancing effect in combination with +, it does inhibit the effect of RpII215⁴ at 29^oC but not at 25^oC or 19^oC. temperature-sensitive lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S1

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S2

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S5

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S6

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S8

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S7

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S11

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S12

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^M

(Kim et al., 1994)

Polr2A^K1 has lethal phenotype, suppressible by Polr2B^S

(Kim et al., 1994)

Polr2A^K1 has lethal | heat sensitive phenotype, suppressible by su(RpII215)S3^S3/su(RpII215)S3^S13

(Mortin, 1990)

Other

Statement

Reference

Marcal1^del, Polr2A^K1/RpII215[+] has partially lethal - majority die | embryonic stage phenotype

(Baradaran-Heravi et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Polr2A^K1 has phenotype, suppressible by Polr2B^SM

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S8

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by su(RpII215)S3¹

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by su(RpII215)S3²

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^M

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S11

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S12

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S1

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S2

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S5

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S6

(Krasnoselskaya et al., 1998)

Polr2A^K1 has phenotype, suppressible by Polr2B^S7

(Krasnoselskaya et al., 1998)

Polr2A^K1 has | heat sensitive phenotype, suppressible by Polr2B^S8

(Mortin, 1990)

Suppressor of

Statement

Reference

Polr2A^K1 is a suppressor of phenotype of Polr2B^SM

(Krasnoselskaya et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Marcal1^del/Marcal1^del RpII215^K1/+ embryos show a reduced hatching rate compared to controls at 20[o]C.

(Baradaran-Heravi et al., 2012)

Flies carrying RpII215^K1, RpII140^A5 and RpII140^SM are viable: i.e. RpII215^K1 and RpII140^SM show reciprocal suppression.

(Krasnoselskaya et al., 1998)

The recessive lethality of RpII215^K1 is rescued to more than 50% viability by one copy of RpII140^S1, RpII140^S2, RpII140^S5, RpII140^S6 or RpII140^S8. The recessive lethality of RpII215^K1 is rescued to between 1 and 49% viability by one copy of RpII140^S7, RpII140^S11 or RpII140^S12. The recessive lethality of RpII215^K1 is suppressed by RpII140^M or RpII140^S.

(Kim et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

34758

ras¹ v¹ Polr2A^K1; Polr2B^S11

34759

ras¹ v¹ Polr2A^K1; Polr2B^S12

34756

y¹ w¹ Polr2A^K1 m¹ f¹/FM7c

606944

y¹ w^* Polr2A^K1 m¹ f¹/FM7a; P{Polr2A.Halo}attP40

Notes on Origin

Discoverer

Kim.

Comments

Postulated to be defective in elongation or termination.

(Krasnoselskaya et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Polr2A^K1

RpII215^K1

(Baradaran-Heravi et al., 2012)

RpII215^WJK1

(Mortin, 1990)

WJK1

(Chen et al., 1993, Mortin et al., 1988)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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