FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\spo1
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General Information
Symbol
Dmel\spo1
Species
D. melanogaster
Name
FlyBase ID
FBal0016044
Feature type
allele
Associated gene
Dmel\spo
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Amino acid replacement: I44T.

(Namiki et al., 2005)

Nucleotide substitution: C132T.

(Namiki et al., 2005)

Amino acid replacement: R138A.

(Namiki et al., 2005)

Nucleotide substitution: G414A.

(Namiki et al., 2005)

Amino acid replacement: F167S.

(Namiki et al., 2005)

Nucleotide substitution: T500C.

(Namiki et al., 2005)

Amino acid replacement: P203A.

(Namiki et al., 2005)

Nucleotide substitution: C608A.

(Namiki et al., 2005)

Amino acid replacement: S425N.

(Namiki et al., 2005)

Nucleotide substitution: G1274A.

(Namiki et al., 2005)

Amino acid replacement: G481E.

(Namiki et al., 2005)

Nucleotide substitution: G1442A.

(Namiki et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:5,590,777..5,590,777 [-]
Nucleotide change:

G5590777A

Reported nucleotide change:

G1442A

Amino acid change:

G481E | spo-PA; G481E | spo-PC

Reported amino acid change:

G481E

Comment:

Point mutation in an evolutionarily conserved heme-binding domain. 5 other amino acid changes were reported in the mutant but some of them do not result in the reported amino acid changes. This one is highlighted as a significant change.

(Namiki et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      7 to 16 hour homozygous embryos contain very low concentrations of both ecdysone and 20-hydroxyecdysone compared to control embryos. Mutants fail to make cuticle and show defects in morphogenetic movements during late embryogenesis that lead to a failure of head involution and defective midgut formation.

      (Chavez et al., 2000)

      Larval mouth parts and denticles not differentiated.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      spo1 has lethal phenotype, suppressible by Scer\GAL4Act5C.PU/Bmor\Cyp307a1UAS.Tag:HA

      (Namiki et al., 2005)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Bmor\Cyp307a1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PU rescues the lethality of spo1 homozygotes.

      (Namiki et al., 2005)
      Complementation and Rescue Data
      Rescued by

      spo1 is rescued by Scer\GAL4Act5C.PU/spoUAS.Tag:HA

      (Namiki et al., 2005)
      Comments

      Expression of spoScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PU rescues the lethality of spo1 homozygotes.

      (Namiki et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      spo1
      (Tesson and Vincent, 2025, Dib et al., 2021, Chanut-Delalande et al., 2014, Namiki et al., 2005)
      spo4G9
      spo4G
      (Tearle and Nusslein-Volhard, 1987)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)