FlyBase Allele Report: Dmel\tll[l49]

General Information

Symbol

Dmel\tll^l49

Species

D. melanogaster

Name

FlyBase ID

FBal0016893

Feature type

allele

Associated gene

Dmel\tll

Associated Insertion(s)

Carried in Construct

Also Known As

tll¹⁴⁹, tll^I49, tll⁴⁹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Diaz et al., 1996, Pignoni et al., 1990)

Progenitor genotype

Cytology

Description

Nucleotide substitution: T?A.

(Diaz et al., 1996)

Mutation results in a protein that terminates in the second zinc finger at the Leu just before the second Cys.

(Diaz et al., 1996)

Amino acid replacement: C78term.

(Diaz et al., 1996)

Point mutation.

(Margolis et al., 1995, Pignoni et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:30,852,901..30,852,901 [+]

Nucleotide change:

T30852901A

Reported nucleotide change:

T596A

Amino acid change:

C78term | tll-PA

Reported amino acid change:

C78term

(Diaz et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment 8

(Diaz et al., 1996)

anterior protocerebral neuromere

(Younossi-Hartenstein et al., 1997)

Bolwig organ

(Daniel et al., 1999)

corpus cardiacum primordium

(De Velasco et al., 2004)

embryonic/larval hindgut | embryonic stage

(Diaz et al., 1996)

embryonic/larval mushroom body

(Pereanu et al., 2007)

embryonic/larval optic lobe

(Daniel et al., 1999)

embryonic brain

(Curt et al., 2019, De Velasco et al., 2004)

embryonic central brain

(de Velasco et al., 2007)

ganglion mother cell | embryonic stage

(Curt et al., 2019)

ganglion mother cell | somatic clone | third instar larval stage

(Kurusu et al., 2009)

larval protocerebrum

(Pereanu et al., 2007)

neuroblast | embryonic stage

(Curt et al., 2019)

neuroblast MBp | somatic clone

(Kurusu et al., 2009)

pars intercerebralis primordium

(de Velasco et al., 2007)

posterior protocerebral neuromere

(Younossi-Hartenstein et al., 1997)

protocerebrum

(de Velasco et al., 2007)

type II neuroblast | embryonic stage

(Curt et al., 2019)

Detailed Description

Statement

Reference

tll^l49 mutant embryos have significantly reduced proliferation of neuroblasts and daughters and significantly reduced numbers of neuroblasts, including type II neuroblasts, in the brain, compared to controls.

(Curt et al., 2019)

MARCM clones of tll^l49 in the third instar mushroom body (MB) show a reduction in the number of labelled neurons to 8.7, compared to 200 in controls. In adults, this number barely increases (10.4, compared to >500 in controls), and the axonal projections of these mutant clones are predominantly restricted to the gamma lobes, but a few enter the alpha-prime and beta-prime lobes.

tll^l49 mutant MB clones are not accompanied by identifiable neuroblasts at 40 hours after puparium formation (APF), whilst controls are associated with three or four even at 60 hours APF. There is a marked reduction in the incorporation of BrdU in tll^l49 mutant MB clones at both the larval and pupal stages compared to controls, and most of the BrdU labelling is lost after a thirty two hour pulse-chase. Cell death is markedly increased in third instar larval ganglion mother cells in tll^l49 MB clones compared to controls.

(Kurusu et al., 2009)

Single cell class IV dendrite arborisation (da) neuron clones that are homozygous for tll^l49 do not show defects in the establishment or maintenance of dendritic tiling.

(Parrish et al., 2007)

Mutant embryos lack the Fas2-positive protocerebral neuropile. ey-positive mushroom body cells seen in the dorsal protocerebrum of wild-type embryos are also missing in the mutant embryos.

Mutant embryos ar unable to hatch, but stay alive for up to 24 hours under oil.

Late stage mutant embryos move incessantly (wild-type embryos from stage 17d onwards show phases of movement that alternate with phases of complete stillness). The movement pattern of the mutant embryos can be best described as an uncoordinated contraction of individual segments. Segments at different positions along the antero-posterior axis frequently contract at the same time. Left and right hemisegments are often out of phase, and the mutant embryos undergo a high frequency "wiggling" motion. Peristaltic waves are all but absent in the mutant embryos.

(Pereanu et al., 2007)

tll^l49 mutant stage 12 embyro dorsal head exhibit removal of most of the protocerebrum, including the pars intercerebralis.

(de Velasco et al., 2007)

The brain is strongly reduced in mutant stage 16 embryos, the stomatogastric nervous system is intact and the corpus cardiacum is absent.

(De Velasco et al., 2004)

The optic lobe placode appears to invaginate normally in mutant embryos. Late embryos show transformation of the optic lobe into Bolwig's organ; the number of cells in the Bolwig's organ is increased by a factor of 2-3 compared to wild type, while the optic lobe is absent.

(Daniel et al., 1999)

Embryos lack all protocerebral neuroblasts of the anterior (Pa) and posterior (Pp) groups, while most central protocerebral (Pc) neuroblasts segregate normally. The protocerebrum is almost completely missing in late embryos.

(Younossi-Hartenstein et al., 1997)

Embryos lack abdominal segment 8 and have little or no hindgut.

(Diaz et al., 1996)

Strong tll allele.

(Margolis et al., 1995)

tll^l49/Df(3R)tll-pgx embryos lack A8 and filzkorper.

(Steingrimsson et al., 1991)

Cuticular and brain defects.

(Pignoni et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

pros¹⁷, tll^l49 has abnormal neuroanatomy phenotype

(Kurusu et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

tll[+]/tll^l49 is an enhancer of terminalia phenotype of ken^ok

(Lukacsovich et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

tll^l49 pros¹⁷ double mutant third instar larval mushroom body clones show reduced numbers of neurons (approximately 18.6) compared to controls (approximately 200 neurons) (this resembles a tll loss of function phenotype), while tll^l49 pros¹⁷ double mutant non-mushroom body neuroblast clones show a tumour phenotype (this resembles a pros loss of function phenotype).

(Kurusu et al., 2009)

The frequency of terminal defects in ken^ok homozygous adults is increased by tll^l49/+.

(Lukacsovich et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tll^l49/tll^a is rescued by tll^+t10.2

(Pignoni et al., 1990)

tll^l49 is rescued by tll^+t10.2

(Pignoni et al., 1990)

Comments

Mutant phenotype can be rescued to adulthood by P element mediated transformation of the wild type tll gene copy.

(Pignoni et al., 1990)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7093

cu¹ tll^l49/TM3, P{ftz-lacC}SC1, Sb¹ Ser¹ ry^RK

Notes on Origin

Discoverer

Comments

Strong tll allele.

(Pignoni et al., 1990)

Severity of the embryonic phenotype (deletion of either external structures, A8 or anal pads, or internal structures, hindgut) places tll alleles in the order: Df(3R)tll-pgx > tll^l49 > tll¹ > tll^l29 > tll² > tll^le3.

(Diaz et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

tll L49

(Pereanu et al., 2007)

tll¹⁴⁹

(Herrero et al., 2014, Sprecher et al., 2007, Hartmann et al., 2001, Steingrimsson et al., 1991)

tll⁴⁹

(Rives-Quinto et al., 2020, Johns et al., 2018)

tll^49l

(Mishra et al., 2018)

tll^I49

(Konstantinides et al., 2022, Curt et al., 2019)

tll^L49

(de Velasco et al., 2007, Parrish et al., 2007, De Velasco et al., 2004, Liu and Lengyel, 2000, Kuhnlein et al., 1997, Vorbruggen et al., 1997, Younossi-Hartenstein et al., 1997)

tll^l49

(Hakes and Brand, 2020, Jussen et al., 2016, Guillermin et al., 2015, Kurusu et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (28)

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